These results further extend our knowledge of the pathogenicity of t . canis embryonated eggs 本结果进一步扩展了对于犬蛔虫受孕卵致病性的知识。
2.
This is the first report that larvae hatched from t . canis embryonated eggs maintained for a long time can cause murine ocular toxocariasis 本报告为首篇关于自长期培养的犬蛔虫卵孵育的幼虫可引起鼠眼部犬蛔虫症的研究。
3.
The a / pr / 8 / 34 ( hln1 ) adapted to mouse lung was propagated in allantoic cavity of 9 - day - old embryonated chicken eggs and titered by hemagglutinin test , and the tilers was 1 : 320 用9日龄鸡胚培养人流感病毒的鼠肺适应株户jp侧8 / 34 ( hini ) ，通过血凝实验测得病毒滴度为1 : 320 。
4.
Two ndv strains , lasota and f48e9 , were propagated in 9 - day - old embryonated specific - pathogen - free ( spf ) eggs respectively for 36 hours at 37 , then these chicken embryos were placed at 4 for 12h before the allantoic fluid was gained 首先，将ndvlasota株和f48e9株分别接种于9日龄spf鸡胚，于37孵化36h ，置4 12h后收获鸡胚尿囊液。
5.
The rna genome of virus used for rt - pcr can be isolated not only from the infected chicken embryonated fluids , but also directly from tissue homogenate of field samples . the detection results of 67 field samples were identical with that of the conventional diagnosis 本方法只需一个rt - pcr反应，整个过程在8小时内完成，既可取感染鸡胚尿囊液检测， 2002年）丫h人《 ‘ wif寸它义：也可从病料直接检测。
6.
Ocular injury to icr mice from embryonated eggs of toxocara canis , which had been maintained in 2 % formalin for 14 months at 4 c , was evaluated by microscopic and pathologic assessments at 1 , 2 , 3 , 5 , 7 , 28 , 56 , 84 , 112 , 140 , 168 , 196 , 294 and 469 days post infection ( dpi ) 摘要本研究探讨将储存于2 %福马林液内，于4 c下保存14个月之犬蛔虫受孕卵感染小鼠第一天至第四百六十九天后，藉显微镜检视与病理学的研究评估孵化的幼虫对小鼠的眼部损伤情形。
7.
Newcastle disease virus ( ndv ) strain 695 , a thermostable nature avirulent strain , were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid . referred to the reported sequence of f gene , a pair of primers were designed and synthesized . f gene of ndv b95 strain was amplified by rt - pcr , the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method 利用从国外引进的新城疫热稳定性天然弱毒b _ ( 95 )株接种spf鸡胚繁殖病毒，经处理后提取病毒的基因组rna ，参考国内外发表的ndv融合蛋白基因序列，设计一对特异性引物，经反转录聚合酶链式反应( rt - pcr )扩增出约1700bp大小的特异性片段，将此片段回收纯化后，利用t - a克隆技术将其克隆到pgem - t - easy克隆载体中，再转化大肠杆菌jm109感受态细胞，转化后经分子量比较、 pcr鉴定和酶切分析筛选阳性克隆。
8.
Materials and methods : fecal , cloacal and tracheal swabs from different types of poultry were collected in 6 live - bird retail markets once a week . they were inoculated into 9 - 11 days embryonated chicken eggs and incubated in 35 " c for 72 hours . hi and nl assays were performed to detect the subtype of viruses if ha test were positive ( ad ^ 16 ) and contamination test were negative 材料和方法：每星期收集一次标本，收集的标本常规处理， 9 - 11日龄鸡胚尿囊腔接种， 35培养72小时，收取尿囊液，血凝阳性( ad 16 )且细菌培养阴性者- 70保存并进一步做hi ， ni实验鉴定亚型。
9.
The information generated in current study suggests that the developing influenza ecosystem in southern china region may play an important role in the process of emerging novel influenza viruses , even directly impact the genesis of pandemic influenza strains . materials and methods : fecal , cloacal and tracheal swabs from different types of poultry were collected in live - bird retail markets once a week . they were inoculated into 9 - 11 days embryonated chicken eggs and incubated in 35 for 72 hours 本课题意在: 1 、以pa和cu为代表，探讨hgnz亚型流感病毒在这些新形成的小种群中的流行情况; 2 、探讨这些小种群中流感病毒的来源:是从其它动物跨种属传递而来，还是本身为流感病毒的天然宿主: 3 、探讨这些新形成种群中hgnz亚型流感病毒的进化情况，以及其在整个流感病毒生态体系中的作用;材料和方法:每周采集标本一次，常规处理后，接种于9一n日龄鸡胚尿囊腔， 35恒温培养72小时，收取鸡胚尿囊液。
