| 1. | The recornbinant vector pcambia 1305 . 1 containing s gene was confirmed by double digestion of restriction endonuclease
经双酶切鉴定证实s基因克隆到载体pcambia1305 . 1上。 |
| 2. | The positive clone was screened and identified by pcr and analysis of restriction endonuclease digestion
转化感受态大肠杆菌dh5 ，经pcr及限制性内切酶分析得到阳性克隆。 |
| 3. | The pcr product was cloned into pmd18 - t vector . the positive recombinant clone was identified by pcr and endonuclease digest
提取重组质粒经pcr鉴定和酶切鉴定后，对插入片段进行序列测定及分析。 |
| 4. | The deduced amino acid sequence of hau3r protein suggested that propablely it is a kind of endonuclease similar to ea59
根据其核苷酸序列推译的氨基酸序列暗示hau3 ~ r蛋白可能是一种类似于ea59的核酸内切酶。 |
| 5. | 3 . pbv220 - endostatin was transformed into e . coli dh5a . the positive colony was screened and identified by pcr and restriction endonuclease digestion
3 .将重组质粒转化到克隆菌dh5a中，经pcr筛选和限制性内切酶鉴定，得到阳性克隆菌株。 |
| 6. | The genome could be digested only by restriction endonucleases hind , accl and xbal , and was resistant to cleavage by a wide range of other restriction endonucleases
病毒的基因组可以被hind 、 acc和xba酶切消化，但不能被其他所使用的限制性内切酶消化。 |
| 7. | Using recombination techniques , the gene fragment ced - 9 , derived from pbs , was incorporated into pgem - 7zf ( + ) and formed a new vector pgem - ced9 , in order to get the right endonuclease sites
从质粒pbs中获取ced - 9基因片段，构建中间载体pgem - ced9以获得合适的酶切位点。 |
| 8. | The genomic dna of ginkgo extracted by ctab method is light brown in color . it is neither digested with restriction endonucleases nor acted as template for polymerase chain reaction ( pcr )
常规的ctab法提取的银杏基因组dna呈黄色，不易被内切酶所消化，也不能作为pcr模板。 |
| 9. | E2 gene was cut with restriction endonuclease from the recombination plasmid t - e2 , and subcloned into the expression vector ppic9 ' s mcs , which was identified by enzyme digestion and pcr amplification
将重组质粒t - e2的e2基因酶切后，亚克隆到表达载体ppic9上，经酶切和pcr鉴定，命名为ppic9 - e2 。 |
| 10. | The recombinant vector pbi121 containing si gene of ibv was confirmed by pcr and double digestion of restriction endonuclease . agrobacterium fumefaciens eha105 with the recombinant vector pbi121 was obtained by tri - parental mating method
用三亲交配法将重组克隆导入根癌农杆菌，获得了含pbi121重组质粒的eha105农杆菌菌株。 |
