The cbd tag of the fusion protein was cut by site - specific protease enterokinase at starting met of the target protein lt 27 . it was released from the cbd fusion tag efficiently 利用融合蛋白中目的蛋白lt 27与cbdtag接头处的肠激酶特异性识别位点，用肠激酶处理粗纯化的融合蛋白，可将卜
3.
Because of the cleavage site of enterokinase and cnbr was designed in the middle of thioredoxin and cmiv , the expressed peptides of the mutation of cmiv could be cuted down from the fusion protein by enterokinase or cnbr 由于硫氧还蛋白和抗菌肽之间设计了肠激酶( enterokinase )切割位点和cnbr切割位点，通过对该表达的融合蛋白的切割，可得到目标抗菌肽cmiv突变体多肽分子。
4.
The sds - page results showed the fusion protein was efficiently expressed in the soluble form . 3 ) the expressed fusion protein was purified and cleavaged by enterokinase to release the mutation i of cmiv and the mutation ii , whiches exhibited antibacterial activity to the ecoli . k12 三、对以上表达的融合蛋白进行初步纯化以及利用enterokinase切割融合蛋白，并进行抗菌活性检测，结果表明所设计的cmiv突变体具有抗菌活性。
