| 1. | Relationship of serum activin a and follistatin in pregnant women
及卵泡休止素水平变化的关系 |
| 2. | The purified bovine follistatin cdna was inserted into pet - 30a vector to construct the prokaryotic expression vector pet - 30a - fs2
经正反测序表明其与genbank中发表的牛follistatincdna序列完全相同。 |
| 3. | Bovine follistatin cdna gene was cloned by rt - pcr using total rna as template , which was extracted from bovine ovary by trizol total rna extract kit
本实验从牛的卵巢中用trizol提取总rna ，并通过rt - pcr (反转录pcr )的方法扩增获得follistatincdna 。 |
| 4. | To integrate my work to other researches in our laboratory on myostatin gene , the purpose of my research is cloning and prokaryotic expression of the bovine follistatin cdna gene
为了与实验室进行的对myostatin的研究结合，本实验进行了牛follistatincdna的克隆及原核表达。 |
| 5. | Follistatin may have pharmacological applications for treating human muscle diseases as well as applications as an agent for enhancing muscle growth of livestock
Follistatin今后可能会用于治疗人类肌肉性疾病，以及提高家畜肌肉的质量和数量，本实验为这方面的研究提供了一定的实验基础。 |
| 6. | Follistatin antagonises myostatin by a direct protein - protein interaction , which prevents myostatin from executing its inhibitory effect on muscle development
Myostatin是目前所知的最强的肌肉抑制因子， follistatin通过直接的蛋白结合而拮抗myostatin对肌肉发育的抑制作用，表现出增肌效应。 |
| 7. | Sequence analysis data indicated that 99 % homology was indentified between the published bovine follistatin cdna gene and my two - directional sequenced products . the sequence data also shows that the open reading frame of the pet - 30a - fs2 is correct
将获得的follistatincdna基因克隆入原核表达载体pet - 30a中，组装成了原核表达载体pet - 30a - fs2 ，经正反测序表明其阅读框正确。 |
| 8. | Follistatin inhibits myostatin ' s ability to bind to receptors and has applications for promoting muscle growth by a combined function of muscle fiber hyperplasia ( the number of muscle fibers ) and hypertrophy ( the thickness of fibers )
Follistatin增肌效应并非病态的肌肉增长，对其它的组织与器官亦无有害的影响，这在畜牧业的生产上对提高家畜的肉用性状有着深广的实用意义。 |
| 9. | Follistatin , a kind of secreted protein , is known to antagonise the function of several members of the tgf - beta ( transforming growth factor - beta ) superfamily including myostatin , the most powerful inhibitor of muscle growth characterised to date
卵泡抑素( follistatin )是一种分泌蛋白，它可以拮抗tgf - beta ( transforminggrowthfactor ，转化生长因子)超家族中几种成员的功能，包括myostatin 。 |
