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Home > chinese-english > "fpv" in English

English translation for "fpv"

飞行航径矢量
家禽疫簿
猫泛白细胞减少症病毒
猫细小病毒
首次通过法心室造影术
细小病毒抗体


Related Translations:
fowl pest virus fpv:  家禽疫病毒
Example Sentences:
1.Fpv flight path vector
飞行航径矢量
2.Construction of recombinant fowlpox virus coexpressing aiv ha and ndv f . for the construction of transfer vector pfgs11haf , aiv ha gene of f strain in puc18ha and ndv f gene of f48e8 strain in puc19f were removed and inserted into pfgs11 . recombinant fowlpox viruses ( rfpv ) coexpressing aiv ha gene and ndv f gene were constructed by using different promoters of ps and pe / l . recombinant rfpvs were derived by dosper liposome - mediated transfection with the two transfer vectors on chicken embryo fibroblast ( cef ) monolayer cultures which were infected by wild type fpv chinese vaccine strain 282e4 3 - 4 hours earlier
Puc18ha和sk质粒同时经hind 、 kpn酶切后连接得中间质粒skha ;将质粒skha用bamhi酶切回收ha基因插入到插入载体pfgs11中的bamhi位点,通过酶切鉴定获得了pfgs11ha ;将含ndvf基因的质粒puc19f用hind 、 sal酶切经klenow酶补平插入到经sma酶切后的skifn中pe / l启动子下获得中间质粒skf ,再将质粒skf和puc18质粒先分别用ecor 、 xho酶切klenow补平,后再共同用sac酶切连接得puc18pelf , sal酶切回收pe / l - f基因盒插入到pfgs11ha的sal位点,通过酶切鉴定获得了pe / l - f与ps - ha同向的表达载体pfgs11haf 。
3.In this study , the recombinant fowl - poxvirus was transfected into expressing the vp3 gene of isolated gpv h1 strain into the cef cells with fpv - 017 by liposome , which have the lacz reporter gene , earlier / latter promoters lp2ep2 of fpv , promoters p7 . 5 and p7 . 1 of vaccinia virus , replication unnecessary region of fpv - 017 . following 6 cycles screenings , clonings , purification of blue plaques , detection of pcr and dot - elisa , which verified the genetic stable vp3 - fowlpox virus recombinant constructed successfully . this study provided the theoretical and practical foundation for development of gpv recombinant fowl - poxvirus genetic engineering vaccine , as well as provided substance preparatory for prevention the high mortality gpv
本研究采用脂质体转染方法,将含有完整gpvh1分离株vp3基因、报告基因lacz 、禽痘病毒早晚期启动子lp2ep2 、痘苗病毒启动子p7 . 5 、 p11和fpv - 017复制非必须区的转移载体质粒psy681vp3lacz与fpv - 017共转染鸡胚成纤维细胞,经6轮蚀斑克隆、筛选、表达, pcr鉴定和dot - elisa检测,证明该重组病毒已构建成功,并获得了遗传性状稳定的鹅细小病毒vp3基因的重组禽痘病毒。
4.Vp3 gene of hl isolate of goose parvovirus derived from recombinant piasmid pproex htb - vp3 was subcloned into ecorl site of psy538 , and the reporter gene lacz with promoter pll was cloned into smai site of recombinant piasmid . both vp3 gene and lacz gene were cloned into noti site of psy681 , recombinan fpv transfer vector containing vp3 gene of gpv was obtained . the result is basis of construction of recombinant fpv expressing vp3 gene of gpv and gpv genetic engineering vaccine
本研究另从含有gpvh1分离株主要结构蛋白vp3基因的重组质粒pproexhtb - vp3中切取gpvh1株vp3基因片段,将其亚克隆于psy538的ecori位点,然后将带有痘苗病毒启动子p11的lacz报告基因平端克隆于上述重组子的smai位点,再用noti切下同时含有vp3基因和lacz报告基因的片段,再亚克隆于psy681的noti位点,构建出含有vp3基因的重组禽痘病毒转移载体,为构建表达vp3基因的重组禽痘病毒从而制备gpv基因工程疫苗奠定了基础。
Similar Words:
"fpt foxpro" English translation, "fpu" English translation, "fpu floating point unit" English translation, "fpum" English translation, "fpushbutton(component)" English translation, "fpva" English translation, "fpw = fire prevention week" English translation, "fpw floorplan plus for windows" English translation, "fpwht=final pwht" English translation, "fpy" English translation