| 1. | Gnt - v has been purified and cloned , but many unknown questions about its function remain to be resolved
本实验室对gnt - v的研究已有多年，但我们发现gnt - v的功能尚有很多问题未解决。 |
| 2. | State of the railway vehicles - control and instrumentation technology - part 207 - 8 : on - board equipment - gnt overspeed control for tilting trains
铁路车辆状况.控制和测量技术.第207 - 8部分:车上设备 |
| 3. | The elucidation of these problems will be of benefit to the understanding of gnt - v function , and will provide valuable information concerning molecular mechanism of tumor metastasis
这些问题的阐明不仅有利于进一步认识gnt - v的功能，而且为肿瘤转移的分子机理提供有价值的资料。 |
| 4. | Gnt - v was reported to overexpress in many malignant tumors , and the increase of its product - 1 , 6 branching is a characteristic of cancer progress and metastasis potential
Gnt - v在很多恶性肿瘤中部有过量表达，其产物复杂型n -糖链1 ， 6glcnac分支的增多可能是肿瘤发展和转移潜能的重要特征。 |
| 5. | Are the changes in signal transduction and gene expression related to the structural alteration of the sugar chain on some surface receptors resulting from the transfection with sense or antisense gnt - v
而细胞信号转导的改变是否与细胞表面某些受体的糖链结构的改变(由gnt - v的正义或反义cdna转染引起的)有关 |
| 6. | Human gnt - v contains 741 amino acids with six potential sites for n - glycosylation and bears high homology to gnt - v of rat . its gene is located on chromosome 2q21 containing 17 exons . gnt - v protein is encoded by exons 2 - 17 as open reading frame
人类gnt - v由741个氨基酸组成，有6个潜在的n -糖基化位点，基因定位于染色体2q21 ，含有17个外显子，其开放阅读框架由外显子2 - 17进行编码。 |
| 7. | Part ii effects of gnt - v on the phosphorylation and activity of egfr signaling molecules in h7721 cells modification of the n - glycan structure and function of egfr by the transfection of different gnt - v cdnas may lead to the alterations of the phosphorylation and activity in the downstream molecules of signal transduction
Gntv一s / h7721细胞p42 / 44mapk的磷酸化也较mock细胞增强，而gntv一as舰7721细胞则减弱，表明egfr的这两条信号通路中信号分子的磷酸化或活力都受gnt一v的影响。 |
| 8. | These results indicate that the alteration of cell proliferation and dna synthesis caused by different gnt - v cdna transfection may at least partly result from the modification of n - glycan structure and function of egfr . it seems that the increased 1 , 6 glcnac branch on the n - glycans of egfr may benefit to its binding with egf and the resulting tyrosine auto - phosphorylatio n , while the decrease of this branch may prevent these processes
用特异性抗体结合westemblot结果发现，正义或反义gnt一vcdna的转染并不引起pkb 、 p44 / 42mapk和mek蛋白质表达的变化，而gntv一s / h ” 21细胞pkbt308 、 5473位点磷酸化和免疫沉淀pkb的酪氨酸磷酸化以及以gsk召a /日磷酸化为指标的pkb的活性都较mock细胞增加， gntv一as / h7721细胞中这些指标的变化则相反。 |
| 9. | Part i effects of gnt - v on cell proliferation , cell sensitivity to egf and the egf receptor of h7721 cell line using mtt method , it was found that the proliferation of cells transfected with sense gnt - v cdna was facilitated , and both of the total 3h - tdr incorporation and the specific incorporation per cell were also increased . oppositely , these parameters were reduced in cells transfected with antisense cdna of gnt - v . these results suggested that cell proliferation and dna synthesis were modulated by gnt - v
第一部分gnt - v对h7721细胞生长、 egf敏感性和egf受体的影响用mtt方法发现转染正义gnt - vcdna的h7721细胞增殖速度加快，而且无论是~ 3h - tdr的总参入量或每个细胞的参入量均见增加，说明dna合成增强，而转染反义gnt - vcdna的h7721细胞则完全相反， dna合成和细胞增殖速度均见降低，这提示gnt - v可调节细胞的生长。 |
| 10. | In the presented study , using h7721 human hepatocarcinoma cell line transfected with sense or antisense cdna of gnt - v , the effects of gnt - v on signal transduction an d its mechanism as well as the alteration of gene expression were investigated . we expected to elucidate whether and how the transfected glycosyltransferase modulate the cell signal transduction and gene expression
本文以稳定转染gnt - v正义或反义cdna质粒的h7721人肝癌细胞为材料，从下列四个方面对gnt - v影响信号转导及其机制以及引起基因表达的变化进行了研究，企图阐明糖基转移酶转染是怎样调节细胞信号转导的 |
