| 1. | Many attempts to transform s . griseus protoplast with plasmid plz107 were unsuccessful
多次尝试用plz107转化链霉菌原生质体未获成功。 |
| 2. | In this study , growth of streptomyces griseus in liquid ympd medium was determined by measuring the od560 at the interval of 1 hour
本研究通过测定菌液的od _ ( 560 ) ，发现并确证了灰色链霉菌存在二次生长现象。 |
| 3. | Ladybird propylaeajaponica & harmonia axyridis , ground beetle pseudoophonus griseus and ant lion chrysopidae were dominant natural enemies migrating with insect pests
龟纹瓢虫propylaeajaponica &异色瓢虫harmoniaaxyridis ,步甲pseudoophonusgriseus和草蛉是伴随害虫迁飞的主要天敌。 |
| 4. | Later , researchers used fermentation to isolate large quantities of vitamin b12 in solution with bacteria streptomyces griseus , which technique is still widely used in the pharmaceutical field
后来，学者更进一步由发酵液中分离出维他命b12 ，而此种发酵液中分离的方法也是药厂工业制造维他命12之主要方法。 |
| 5. | Strain sa - coo by southern hybridization . a cosmid - based gene library of streptomyces griseus atcc14811 was constructed using phz1357 , a streptomyces - e . coli bifunctional vector carrying two cohesive sites
为了获得胆固醇氧化酶基因，以大肠杆菌-链霉菌双功能柯斯质粒phz1357为载体，构建了灰色链霉菌atcc14811的基因组文库。 |
| 6. | An internal segment of the whig gene of s . griseus was amplified from plzl through pcr . the 304bp dna fragment was inserted into the ecori / bamhi site of e . coli - streptomyces shuttle plasmid pkc1139 , generating pkc1139 : : a whig , named plz107 , for gene disruption
Pcr法克隆whig基因内部304bp片段，连接到大肠杆菌-链霉菌穿梭质粒吓0139 ，构建了基因阳坏用重组质粒贼q139 ： ：凸mg ，命名plz107 。 |
| 7. | Fr - 008 and streptomyces griseus imru3570 are two independently isolated producers for a similar heptaene macrolide antibiotic . comparative studies between the two producers at chemical and biological level are the main task of the present work , no obvious difference was observed on the hplc profiles
本文主要从化学和生物学两个角度分析和比较了这两个菌株的异同，并对抗生素的氨基海藻糖残基基因进行了克隆尝试。 |
| 8. | As heterologous probe and subsequently show to code for desired enzymatic activity . after a serial of subcloning coupled with southern hybridization and enzymatic activity assay , the functional s . griseus atcc14811 cholesterol oxidase gene ( chog ) was localized onto 2 . 3kb ecori - sall fragment
对phz1140和phz1141进行bamh及bgl的酶谱分析及与choa探针的杂交，将胆同醇氧化酶基因初步定位在8 . 8kbbamh和9 . 9kbbgl片段上。 |
| 9. | The enzyme retained full activity after being treated at room temperature for 1 hour at ph between 4 . 0 and 11 . 5 . the enzyme can be incubated at 50 for 4h with only less 50 percent loss of activity and is stable in the frozen state . when streptomyces griseus atcc14811 was cultured in 10 . 3 % sucrose yeme liquid medium , production of extracellular cholesterol oxidase increased for 5 days before decrease
利用硫酸铵盐析及deae -纤维素离子交换柱层析提取纯化灰色链霉菌atcc14811发酵上清液中的胆固醇氧化酶，理化性质研究表明酶作用晟适ph为8 . 0 ，最适温度为45 ， ph稳定范围在ph4 . 0 - 11 . 5之间，在50条件下保温4h ，仍保留54酶活力。 |
| 10. | The precursor of cholesterol oxidase of atcc14811 was predicted with a calculated mr of 60020 . the cholesterol oxidase produced by streptomyces griseus atcc14811 was purified from the culture broth by procedures including steps for preparation of crude enzyme solution , ammonium sulfate fraction and column chromatography on deae - cellulose
Chog与chob ( brevibacteriumsterolicum )的氨基酸同一性和相似性分别为53和70 ，根据推导的氨基酸序列chog蛋白质前体的分子量为60020 ， chogn端区域显示信号肽特征。 |
