English translation for "gvbd"

胚泡破裂

Example Sentences:

	1.	The calcium oscillation and distribution before gvbd in mouse oocyte 小鼠卵母细胞生发泡破裂前的钙离子波动及分布
	2.	By nuclear transferee transfered the nih 3t3 cell to enucleated gv and mii oocyte . the immunofluorescence results showed that jak . 2 was not transfered to somatoblast nuclear whether the recombined oocyte occured gvbd or not . when transfered somatoblast nuclear to enucleated and integrated m ii oocyte , the immunofluorescence results showed that jak2 only existed in the nuclear of integrated m ii oocyte but not the somatoblast nuclear . the nuclear transfer implied that the mature and immature oocyte cytoplasm could not transfer the jak . 2 protein to donor nuclear 通过核移植，将nih3t3体细胞核移入去核gv期和m期的卵母细胞中。荧光检测发现，体细胞核与去核gv期卵母细胞质构成的重组卵无论是否发生gvbd ，核内均没有jak2的出现。在去核和未去核的m期卵母细胞中移入体细胞核， jak2仅存在于未去核m期卵母细胞的核内，并不转移到体细胞核内。
	3.	Protein kinase a is camp - dependent kinase , one of the most important signal transduction pathways , plays a pivotal role in growth , differentiation , tumor occur , cell cycle control , etc . pka activators such as camp , 8 - br - camp or phosphodiesterase ( pde ) inhibitor isomethyl butyl xanthine ( imbx ) or purified pka catalytic subunit all can inhibit germinal vesicle breakdown ( gvbd ) and meiotic maturation in mouse oocytes , also in xenopus oocytes 蛋白激酶a ( proteinkinasea ， pka )是依赖于camp的丝苏氨酸蛋白激酶，是重要的信号传导途径之一，广泛参与许多生命过程，包括生长、分化、肿瘤发生、细胞凋亡、细胞周期调控等。以小鼠卵母细胞为实验对象，给予pka激动剂camp 、 8 - br - camp或磷酸二酯酶抑制剂imbx ( isomethylbutylxanthine )均可抑制小鼠卵母细胞的胚泡破裂及减数分裂成熟。
	4.	As the most common used small experimental animals , rat and mouse have inconsistencys in reproduction , especially in gondonatorpin inducing oocyte maturation . our research was undertook to elucidate which kind of gonadotropins play role in oocyte meiotic maturation , the effects of fsh ans lh / hcg on mouse and rat oocyte maturation in vitro were examined under different culture models 在体外自发成熟模型中，大鼠的去卵丘裸卵( do )自发成熟发生要较卵丘包被的卵母细胞( ceo )早， do培养2h时gvbd可达到88 ，而ceo培养2h的gvbd为53 。
	5.	Rat cumulus - enclosed oocyte ( ceo ) and denuded oocyte ( do ) both spontaneously resume meiotic maturation in vitro , when cultured for 2h , the germinal vesical breakdown percentage of do reaches up to 88 % , and ceos get 53 % . oocytes begin to extrude first polar body 1 ( fb1 ) when cultured for 8h . mouse oocyte spontaneous maturation in vitro can be inhibited by 4mm hypoxanthine ( hx ) or 25um 3 - isobutyl - methylxanthine ( ibmx ) . while , 4mm hx can not inhibit the spontaneous maturation of rat oocyte , and the lowest effective concentration of ibmx to inhibit rat oocyle spontaneous maturation in vitro is 50um . treated with the same concentration of ibmx , gvbd percentage of rat ceo is lower than that of do , the results indicat that cumulus cells delay rat oocyte spontaneous maturation and potentate the inhibition effect of ibmx , but it ' s not the case on mouse Ceo和do培养8h时都开始排出pb1 ；在卵母细胞成熟抑制模型中， 4mm次黄嘌呤( hx )和25 m的异丁基甲基黄嘌呤( ibmx )是小鼠卵母细胞成熟的有效抑制剂，但4mm的hx抑制大鼠卵母细胞成熟效果不理想，用hx - m199和hx - mem培养24h ， gvbd率分别为61 ， 81 ，而ibmx有效抑制大鼠卵母细胞自发成熟的最小浓度为50 m ， ibmx可以降低大鼠ceo和do的gvbd率，但ceo中的卵母细胞gvbd率要显著低于do的gvbd率， 100 m的jbmx处理24h后，大鼠ceo和do的gvbd率分别为24 ， 84 ，提示卵丘细胞的存在可以延迟大鼠卵母细胞的自发成熟，有助于抑制卵母细胞成熟，但在小鼠上ceo和do的自发成熟差异不明显。

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