Elimination of hematoxylin sediments in he sections 切片中苏木素沉渣去除方法探讨
2.
Cells take diverse shapes . these are epithelial cords of block - like cells . as always , nucleoli and nuclear heterochromatin stain darkly with hematoxylin 不同形态的细胞。这些是立方细胞排列成的上皮索状结构。总之，核仁与细胞核中的异染色质被苏木精染成深色。
3.
Finally , the sections were counterstained with hematoxylin , dehydrated and cover - slipped . statistical analysis the data analysis were performed using spss for windows 10 法医病理学上关于icam ?一的研究已有很多，主要集中在应用icamj判定心肌缺血及脑损伤时间可行性方面。
4.
In order to understand the mechanism of spermatozoa living in the spermatheca after copulation , hematoxylin - eosin dyeing method is used to discover the microstructure of the spermatheca by light microscope 为进一步了解雌雄个体交配后精子在受精囊内的存活的机制，采用h - e法，在光镜水平上研究了受精囊的显微结构。
5.
In this paper , the methods that the author used are as follows : light microscopy : the testis was fixed in bouin ' s fluid , dehydrated in an ethyl alcohol series , embedded in paraffin , sectioned at 6 u m and stained with hematoxylin and eosin , then observed with olympus microscopy and photographed 光镜样品以bouin ' s固定液固定，系列酒精脱水，石蜡包埋，切片厚度6 m ，苏木精、伊红染色， olympus显微镜观察并拍照。
6.
The early embryo were made into a series of continuous section slides by tissue cutting . the sections were stained by hematoxylin and eosin ( h & e ) staining and then the development of internal organs such as heart in early embryos was observed by microscope . we found that there is certain relationship between external and internal malformation 同时我们收集人类药物流产的早期胚胎，观察发现胚胎畸形占17 . 86 % ，早期致死占32 . 54 % ;采用组织切片技术将胚胎制成一系列石蜡连续切片，染色后显微观察畸形和正常的早期胚胎内部心脏等器官的发育情况，发现胚胎外部畸形与体内畸形存在一定关联，对此我们将做进一步的研究。
7.
Section two the evaluation of biocompatibility of the acellular dermal matrix by the method of cell culture . the new born rat ' s epdermic cells were cultured with the acellular dermal matrix together as experiment group , while the epdermic cell were cultured simply as control . 24 hours later , under the invert microscope , the epidermic cells anchored well and transparent flat cells were observed in both groups . 7 days later , both cultured cells were taked out and fixed in 95 % ethanol , stained with hematoxylin and were observed under light microscope . many cleaved cells were observed in both groups . during cell culture , no pathogenic microganism was observed . so we considered the acellular dermal matrix was aseptic and had good biocompatibility . section three subdermal implantation of the acellular dermal matrix . 24 rats were used in the experiments . a piece of acellular dermal matrix ( 1 . 5 x 1 . 5cm2 ) was implanted beneath the dorsum skin flaps of each rat , 1 week , 2 weeks , 3 weeks and 4 weeks after implantation , 6 pieces of acellular dermal matrix were harvested and the size of implanted acellular dermal matrix were measured , the sections were used for he staining and observed under light microscope . the result were as folio wing : 1 - 2 weeks after implantation , the acellular dermal matrix began to adhere to the tissue around and turned red gradually ; 3 - 4weeks after implantation , the acellular dermal matrix adhered closely to the tissue around and could be recognized easily , 1 - 3 weeks after implantation , the size of implanted acellular dermal matrix had no statistical difference ( p > 0 . 05 ) . 4 weeks after implantation implanted acellular dermal matrix contracted ( p < 0 . 05 ) . under light microscope , l - 2weeks after implantation , the fibroblast cells infiltrated the acellular dermal matrix and a small amount endothelial cells of vessel and lympho - histiocytic cells infiltrated the acellular dermal matrix . 3 - 4 weeks after implantation , infiltrating blood vessels were evident . so we think that the acellular dermal matrix had low immunological reactions and could induce the infiltration of fibroblast macrophage cell and the endothelial cells of vessel 结果如下：皮下包埋卜周者，无细胞真皮基质渐与周围组织粘附，颜色由苍白转红；皮下包埋3周者，无细胞真皮基质与周围组织紧密枯附，盾晰叶辩；术后卜周，包埋的基质面积变化较包埋前无统计学差异o川0引，术后4周包埋的无细胞真皮基质面积较包埋前缩小j刃刀5 ） 。光镜下术后卜周，宿主的淋巳组织细胞、成纤维细胞浸入生长，釉附在胶原纤维上，少量血管内皮细胞浸入基质；术后34周，无细胞真皮基质内较多的血管形成，故可认为无细胞真皮基质免疫原性低，能诱导宿主的成纤维细胞、巨噬细胞浸入生长，为一种新型的真皮替代物。第四部分无细胞真皮基质与自体断层皮片复合移棺的研究， sd大鼠10只，在其背部卜方造成全厚皮肤缺损的创面
