| 1. | This structure increased obviously following hyperosmotic stimulation
高渗刺激后， edas的数量明显增多。 |
| 2. | While it is not clear that whether son astrocytes respond to hyperosmotic stimuli and what ' s the relationship between them and neurons
但是，关于胶质细胞在高渗刺激状况下，是否参与渗透压的调节及其与神经元的关系的研究尚未见报道。 |
| 3. | Treat primary cultured neurons and astrocytes with hyperosmotic stimulation . immunofluorescence was used to study the expression of cx43 and cx32 in son neurons and astrocytes
应用westernblot技术检测高渗刺激后视上核缝隙连接蛋白（ connexin ， cx ） 32和43含量。 |
| 4. | After hyperosmotic treatment , cx43 - li granules increased quickly on the plasm of the cultured astrocytes and cx32 - li increased apparently in the cultured neurons
而对培养的神经元和星形胶质细胞施予高渗刺激，星形胶质细胞膜上cx43样阳性颗粒迅速增多，神经元cx32表达明显增强。 |
| 5. | Objective : in this study we try to simultaneously investigate the response of neurons and astrocytes of rats following hyperosmotic stimulation and focus attention on the role of neuronal - astrocytic complex in the osmotic reglution
目的：本研究试图从整体水平共同观察神经元和ast对高渗透压刺激的反应，探讨神经胶质细胞?神经元复合体在渗透压调控过程中的作用。 |
| 6. | 5 , after being treated with hyperosmotic stimulation , the cultured astrocytes and microglias showed a fast increase of intracellular calcium concentration followed by a decrease . the [ ca2 + ] j of astrocytes decreased slower than that of microglias . according to the r
根据实验结果，得出以下结论： l 、高渗刺激可以引起大鼠son内的星形胶质细胞和小胶质细胞快速的反应，星形胶质细胞的反应要早于神经元。 |
| 7. | Based on the previous researches , the present study investigated the response of son neurons and glias ( astrocytes and microglias ) to hyperosmotic stimulation and their relationship to find out the roles of glias in regulating hyperosmotic stimulation
因此在以往研究的基础上，本课题通过观察视上核神经元和胶质细胞(星形胶质细胞和小胶质细胞)对高渗刺激的反应及其相互关系，探讨视上核胶质细胞在高渗刺激后调节过程中的作用。 |
| 8. | Conclusions : the present results indicated that the brain areas involving in the heperosmotic regulation mainly located in mvz son and pvn . the neurons and astrocytes might be very active following hyperosmotic pressure and n - asc as a functional unit might serve to modulate osmotic pressure . there was reciprocal osmoregulation pathways between the mvz and son or pvn in the brain
结论：参与大鼠高渗性渗透压调节的中枢主要位于mvz 、 son和pvn等核团，神经元和ast以n asc的形式共同参与渗透压调节反应，体内存在mvz和son或pvn之间往返的渗透压调节通路。 |
| 9. | Methods : hyperosmotic pressure animal model was established by administering 3 % sodium chloride as drinking water to rats or increasing osmotic pressure of the culture medium . osmoregulation positions in the brain , reciprocal projection pathways between the medullary visceral zone ( mvz ) and supraoptic nucleus ( son ) or hypothalamic paraventricular nucleus ( pvn ) , oscillation of intracellular calcium in cultured neurons and astrocytes were studied by means of anti - fos , glial fibrillary acidic protein ( gfap ) , tyrosine hydroxylase ( th ) or vasopressin ( vp ) multiple imrnunohistochemical staining , immuno - electronic microscope , wga - hrp retrogradely tracing and cell culture methods . results : ( 1 ) fos positive neurons within the mvz , parabrachial nuclei , locus ceruleus , pvn , son , subfomical organ increased markedly
方法：通过给予大鼠饮用3氯化钠或提高培养基渗透压浓度的方法复制高渗刺激模型，主要采用抗fos 、胶质原纤维酸性蛋白( gfap )和酪氨酸羟化酶( th ) (或加压素? vp )免疫组织化学多重染色、免疫电镜、 wga - hrp束路追踪结合免疫组织化学多重染色、细胞培养等实验方法，系统观察了中枢参与渗透压反射的调控部位、下丘脑视上核( son )神经元? ast超微结构的变化、延髓内脏带( mvz )和son及下丘脑室旁核( pvn )之间往返投射通路和神经元的性质及其与ast的关系、培养神经元和ast内钙波的变化。 |
| 10. | An acellular dermal matrix was prepared from allogenic skin by removing epidermis with a hyperosmotic salt solution and cross - linking with glutaralaldehyde , then clear away acellular components in dermis with naoh - maceration . the light and sme observation of the acellular dermal matrix revealed that the epidermis and cellular component in dermis were eliminated
本研究共分四部分：第一部分无细胞真皮基质的制备用高渗盐除去异体皮肤的表皮细胞，经戊二醛交联后以低浓度naoh消蚀以除去真皮中的所有细胞成分，得到无细胞真皮基质。 |
