| 1. | Of 5 immunogens . groel and ef were reported in other pathogens
其中groel与ef在其他病原菌的抗血清中也存在抗体。 |
| 2. | The construction and screening of cdna expression library of oncosphere of taenia solium and cloning of immunogen gene
文库的构建及免疫原基因的筛选与克隆 |
| 3. | After many times of electrophoresis , the specific protein band of vd - 8 was cutted and the protein was collected as immunogen
在抗原的提取纯化步骤完成之后，要进行单抗的制备步骤。 |
| 4. | All these as a basis study and explore of the choice of immunogen for preventive and therapeutic vaccines of hepatitis b
为乙型刖：炎的预防和治疗性疫苗免疫原的选择进行了成功的初步研究和探讨。 |
| 5. | D . nodosus pili is the main protective immunogen against footrot in ruminants . il - 2 is known to be as an adjuvant in many areas
已有很多实验证明il - 2作为佐剂能提高体液免疫和细胞介导的免疫反应。 |
| 6. | After the produre of extracting and purifying immunogen , the production of monoclonal antibodies was carried out . three balb / c mice , 6 - week old , were used for immunization
首先选择六周龄的balb / c小鼠三只，按照免疫方案进行尾静脉和皮下的注射。 |
| 7. | Results : the 155 - 226 aa of the hnadcs had good flexibility , surface probability , hydrophilicity and high antigenic index , so it was selected as immunogen to inoculate rabbits
结果： hnadc3的第155 226位氨基酸具有良好的柔韧性、表面可及性和亲水性，和较高的抗原指数，因此选用该段氨基酸片段作为免疫原。 |
| 8. | And the positive clones were used as the immunogens to immunize mice . both of the serum and phage peptides were add to measure their effects on the growth of huvec ( human umbilical vascular endothelial cell )
检验这种短肽可否模拟vegf的抗原决定簇诱发机体产生能与vegf结合的抗体，研究小鼠抗血清对huvec (人脐静脉血管内皮细胞)生长的抑制作用以及噬菌体表达的7肽和12肽对huvec生长的抑制作用。 |
| 9. | Purified fusion protein gst - hnadc3 was used as an immunogen to inoculate rabbits and the antibody against the gst - hnadc3 fusion protein was raised , and was purified by gst sepharose 4b affinity chromatography to remove the antibody against gst
Ptg诱导表达，超声破碎细胞后，采用亲和层析方法纯化融合蛋白gst十nadc3 ，并以此为抗原免疫新西兰株白兔制备融合蛋白抗体。应用亲和层析的方法对gst十nadc3融合蛋白抗体进行纯化，以去除抗gst抗体。 |
| 10. | Serious cross reation existed between v . albo - atrum and mv2 , mv3 , mv4 . the other pathogen isolates v31 and v32 also had cross reactions , but the reaction was not serious . because limited number of pathogen isolates were selected , it could not prove that the selected immunogen was widely presentative , more pathogens isolates should be tested to verify the acquired hybridomas cells
5株单抗杂交瘤细胞中没有一株具有种或属的特异性，其中mv2在棉花黄萎病菌若干菌系间的检测表明其能够区分不同的致病类型； mv1和mv4组合检测的结果基本上能将棉花大丽轮枝菌鉴定到种；黑白轮枝菌与mv2 ， mv3 ， mv4的交叉反应比较强烈，其他菌株v3 ， v32有个别的交叉反应，但不强烈 |
