Co - immunoprecipitation kit and multiple tissue membranes were from clontech company . 2 Northern印迹杂交膜和eo一irrununopreeipitationkit购自clonteeh公司。
2.
Sheshberadaran h , et al . antigenic relationship between hantaan viruses analysed by immunoprecipitation . j gen virol , 1988 , 69 : 2645 梁米芳，等.汉坦病毒s基因在大肠杆菌中的表达及其初步应用.中华实验与临床病毒学杂志， 1993 , 7 : 225
3.
Herein , we used cloning , sequencing , northern blotting , in situ hybridization , in vitro translation and co - immunoprecipitation , aimed to investigate the expression of wnk genes and the function of wnk4 kinase 本实验应用克隆、测序、 northern印迹杂交、原位杂交、体外翻译和免疫共沉淀技术，旨在研究wnk基因的表达情况及wnk4激酶的功能。
4.
And also for the establishment of the technique , we were interested in examining whether rad18 plays a role in this ho - induced dsb repair in s . cerevisiae . to measure the association of radls with dsb , we used the chromatin immunoprecipitation ( chip ) 但是具体这一修复复合物的泛素化底物蛋白是什么，它们又具体结合于什么样性质的dna损伤部分，目前并不清楚，而这两点是研究泛素系统在dna修复中的作用的关键。
5.
The mutants were transfected into pc 12 cells respectively to probed the ability respondence to gdnf stimulation and the interaction with ret by immunoprecipitation , c - ret phosphorylation and immunoblotting assays . the main results are as follows : 1 . expression and purification of recombinant rat gfral protein to obtain recombinant gfral and study its biological activity , the cdna encoding the mature rat gfral was isolated using rt - pcr with total rna extracted from newborn s 纯化和复性后的重组gdnf蛋白，可显著增强pc12一gfral一ret工程细胞的存活和分化;对pc12一ret工程细胞没有任何作用;对pc12一gfral的存活和分化作用11中英文摘要显著强于对pc12一ret的作用，但也显著低于对pc12一gfral一ret细胞的作用。
6.
This study demonstrated that the arabidopsis f - box protein coil associated with atcul1 , atrbxl and skpl - like proteins askl and ask2 to assemble scfcoil ubiquitin ligase complexes . also , we found that the atcull component of scfcoil complexes contained two species including atcull and modified atcull . ( 2 ) we found that coil assembled to two separate scfcoil complexes with either askl or ask2 through immunoprecipitation analysis with plant expressing myc - tagged version of ask2 用表达融合蛋白myc - ask2的拟南芥为材料，以- myc抗体进行免疫共沉淀分析发现， myc - ask2蛋白可以与coi1蛋白一起免疫共沉淀，但是不能与ask1蛋白免疫共沉淀，表明coi1蛋白与ask2蛋白，但是不能同时与ask1结合形成scf ~ ( coi1 )复合体。
7.
However , it is necessary to acquire the antibody or the antiserum , which could specially react with the expression protein of die objective gene transferred into the transgenic plant according to the characteristics of high homology and immune cross - reaction among plant ferritin , using the special immune serum of pea ferritin , the content of plant ferritin could be detected for studing the ferritin expression of transgenic plant by the technique of immunoassay such as immunoprecipitation , eljsa and western blotting 利用免疫检测技术进行植物转基因的表达检测是一种简单、灵敏、快速、可靠的方法，但其前提条件是要有与转基因植物目的基因表达的蛋白质发生特异性免疫反应的抗体或抗血清。根据植物铁蛋白之间有高度同源性和交叉免疫反应的特性，利用特异性的豌豆铁蛋白抗血清，就可通过免疫沉淀、 elisa或western杂交等免疫检测方法进行植物铁蛋白含量等的检测，从而更好地进行转基因方面的研究。
8.
The protein product of meq gene was highly expressed in the nuclei of recombinant baculovirus infected sf9 cells when using an anti - meq monoclonal antibody ( mcab ) 23b46 to run the immunofluorescence assay ( fa ) ; the expression quantity and if staining patterns differed with different times post - infection ( pi ) . the results of western blotting and immunoprecipitation test showed there were two specific bands around 60 kd . the results of the study demonstrated that the baculovirus / insect cell system is effective to be used to express nuclear protein of virus 结果发现:本表达系统产生的meq蛋白可被重组痘病毒表达的meq制备的单抗23b46所识别;在感染细胞中， meq蛋白仅局限于细胞核内，而且随着感染后( pi )时间的增加，具有从核质向核仁和核膜转移的趋向; w已stemblot和免疫沉淀试验均证实重组杆状病毒感染细胞裂解物中出现有两条大小约为60kd的特异带。
9.
Otherwise ; we have used immunoprecipitation and im - munofluores cence microscope technology to study the interaction with doc - 1r and cdk2 proteins under physiological condition in cells , we attempt to find the evidence of interaction with doc - 1r and cdk2 proteins , which will offer experiment basis for understanding the accurate role for doc - 1r gene in cell cycle s regulation 另外我们采用免疫共沉淀技术和免疫荧光显微技术观察在生理条件下细胞内doc - 1r和cdk2蛋白间相互作用的情况，试图找到细胞内doc - 1r与cdk2蛋白结合的证据，为准确掌握doc - 1r基因在细胞周期调控中的作用提供实验依据。
