Cloning and expression of immunotoxin dt389 - hbfgf 免疫毒素的克隆与表达
2.
This result could offer basic informations for estimating the safety of anti - tumor immunotoxin aimed at egfr as target binding site 这一结果为今后对针对egfr的抗肿瘤兔疫毒素进行安全性评价提供了基础资料。
3.
And the expression product was an membrane - toxic immunotoxin . the activity determination in vitro indicated the product had significant killing effect to a43i cell of squamous epithelium carcin 破壁后提取ecoil中的目的产物会由于菌体蛋白的大量搀杂而使提纯步骤繁琐，降低得率。
4.
This project is aimed at : ( 1 ) expressing melittin and megf in e . coli respectively ; ( 2 ) constructing a membrane - toxic immunotoxin by using melittin as toxic part and megf as target part ; ( 3 ) measuring the megf mrna expression abundance by quantitative competitive rt - pcr ; ( 4 ) checking the genetic stability of the recombinant strains constructed in the project ; ( 5 ) optimizing the fermentation conditions of the target strains by flask shake 本课题以大肠杆菌( e . coli )宿主重组表达melittin和megf ；以melittin为毒性部分、 megf为靶向部分，构建膜毒性免疫毒素；用定量竞争rt - pcr法测定megf体内表达丰度；检验所构建的各重组菌株的遗传稳定性；采用摇瓶分析对各重组菌株的发酵条件进行了初步优化。
