The ms188 gene was finely mapped . a total of 8 new indel markers were designed to map msl88 using a segregating population with a total of 2135 male sterile progenies . ms188 was finally mapped to a region of 95 . 8kb between the molecular marker mda7 and k24c1 在与ms188连锁的分子标记mc015附近设计了8个indel分子标记,对遗传群体中2135株不育植株进行基因型分析,最后将目的基因定位于第五条染色体分子标记mda7和k24c1之间95 . 8kb的区间内。