Studies on major parameters of inosine processing 超滤法提取肌苷几个主要参数的研究
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Effect of different organic nitrogen sources on inosine biosynthesis 不同有机氮源对肌苷合成的影响
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In this part the direct neurotrophic effects of inosine were explored 值得注意的是,肌昔的这些作用在其浓度到达1000vm时被逆转。
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Mtt value , morphological characteristics under light and electron microscopy , dna gel electrophresis results , and hoechst33342 dying results show that inosine can protect nscs against apoptosis induced by hioi significantly in a dose dependent manner 2的直接保护作用,通过mtt值的测定,光镜及电镜的形态观察, hoechst33342染色后核形的观察及dna凝胶电泳结果,发现肌昔可对hzoz诱导的c门
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Mmf is the morpholinoethyl ester prodrug of its active metabolite inycophenolic acid ( mpa . mpa is a noncompetitive , reversible inhibitor of the enzyme inosine 5 ' - monophosphate dehydrogenase ( impdh ) , which plays a maor role in the de novo spohesis of guansine nucleotides Balb c和c57bl 6小鼠是纯系的同种小鼠,具有不同的主要组织相容性抗原( mhc i 、 11 ) ,可进行同种排斥反应的兔疫学研究。
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The traditional homogene cloning method was modified on two aspects : first , homo - primers was designed at the sites with minimum degeneracy instead of maximum homology , secondly , some " n " bases were replaced with inosine in degenerate codes , resulting hi the degeneracy reduced from over 1000 times to below 100 times 改进后的同源引物简并度从1000倍以上降低至100倍以下,利用rt一pcr成功地扩增出一条630bp的盐藻cdna片段,该片段与其它物种的烯醇酶基因具有很高的相似性。
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In the second part of this study , the effects of inosine on c17 . 2 in vitro were detected . the results show that inosine can prompt proliferation of c17 . 2 at the range of 0 ~ 800 n m and process outgrowth 50 - 800 m m , also 100 u m inosine can improve the expression level of p - tubulin iii , while at 1000 p m , such effects were reversed 结果显示在0 800vm时,肌昔可明显促进ci7二的增殖,在50 800vm ,可明显促进突起的生长, 100um的肌百可使c厂二p tubulinill的表达上调,对gfap及mag的表达则无影响。