| 1. | Dh5 a at different iptg concentration and different times
Dh5中的表达情况进行检测分析。 |
| 2. | Recombinant proteins were expressed by the hosts after being induced with iptg
实验中选用了两个表达系统，即qiaexpress ( |
| 3. | The positive recombinant plasmid was transformed into e . coli jm109 and induced with iptg
阳性重组质粒转化大肠杆菌jm109 ， ！ |
| 4. | Sds - page analysis showed that the expressed product is a 53kd fusion protein aftor induction with iptg
结果表明，表达产物为53kd的融合蛋白。 |
| 5. | The constructed plasmid containing gst - src fused gene gave a high level of expression of the fused protein
用iptg诱导表达蛋白的产生，纯化后获得大量的目的蛋白。 |
| 6. | The recombinant was transformed into e . co2i ' bl21 strain for expression with the induce of iptg
将表达产物从凝胶中回收，免疫4周龄小白鼠，五次免疫后，采血分离血清。 |
| 7. | The optimum condition was 5 - 6h at immol / l of iptg after long - time induction of recombinant e . coli
综合以上三方面的结果，重组菌pqe16 jm109的最佳的培养条件是lb培养基， ph72 。 |
| 8. | In order to improve the expression level of the protein , the induced temperature and iptg concentration were optimized
为了提高重组蛋白的表达量，对诱导表达时的温度及iptg的浓度进行优化。 |
| 9. | It indicated that optimal induced temperature was 30 c , whereas the change of iptg concentration had little effect
结果表明：最佳诱导温度为30 ，而iptg的浓度变化对重组蛋白的表达量影响不大。 |
| 10. | Then after purification , renaturation , cleavage and ultrafiltration centrifugation , igf - 1 and des ( l - 3 ) igf - l protein were obtained
Westernblot结果表明iptg诱导表达产物可与特异性igf - 1抗体发生反应。 |
