| 1. | Construction and expression of a new human growth hormone isoform - gst fusion protein
人新的生长激素异形体基因的克隆及其在大肠杆菌中的表达 |
| 2. | Physicochemical determinations may include isoform , electrophoretic , and liquid chromatographic patterns , as well as spectroscopic profiles
物理化学性质包括异构体，电泳和液相色谱行为及光谱性质等。 |
| 3. | In cells , apx has several isoenzymes , cytosolic and chloroplastic isoforms are main ones , of which the chloroplastic isoform represents a large portion of apx of the cell
它在细胞内有多种同工酶，主要分为细胞质型和叶绿体型，其中，叶绿体型在细胞中占有很大的比重。 |
| 4. | We have identified p120ctn expression in cultured hepatocyte and liver tissues using rt - pcr , immunohistochemistry and immunofluorescence microscopy , p120ctn isoform 1a and 3 a expression have been detected in the cells and tissues
而当过度表达p120ctn后，细胞粘附能力有所增加，迁移能力降低，并且过度表达的pl加ctn主要分布于膜下，其次是胞浆。 |
| 5. | In other studies of mutant ( knockout ) mice that fail to express the neuronal isoform of nitric oxide synthase ( nos ) , smaller infarcts develop and the mice have less severe neurologic deficits than normal mice ( 61 )
其他一些研究变异（被击昏的）老鼠的试验没有能够表达一氧化氮合酶（ nos ）的神经元对碘氧基苯甲醚，但是出现的梗死梗小，而且与正常的老鼠相比，老鼠的神经学缺陷也不那么严重（ 61 ） 。 |
| 6. | The yeast two - hybrid system was established . in yeast two - hybrid system , mt - 3 interacts with mt - 3 . a further two - hybrid test also indicated that mt - 3 weakly interacted with its isoform mt - 1 . these primary results suggest that mt - 3 probably trend to homodimerize or heterodimerize in vivo
结果表明： mt - 3与mt - 3之间在酵母细胞内能发生相互作用，进一步用酵母双杂交实验还表明mt - 3与mt - 1间也存在弱相互作用。 |
| 7. | Then we cloned the mature ca fragment and the same region of the selected ca fragment from library plasmid to vector pgadt7 , but neither did they interact with ga in the assay of two - hybrid system . then we used arabidopsis cam2 isoform as bait to screen the two - hybrid library
之后将成熟ca和文库中筛到的ca编码部分的基因分别亚克隆至双杂交激活域载体pgadt7 ，进行双杂交检测它们与g蛋白的相互作用，结果仍为阴性。 |
| 8. | In order to understand the apoplast cam gene and the relationship between the apoplast cam and cam isoforms , we intended to get transgenic plants harboring soybean calmodulin isoform genes ( scams ) fused to gfp as a reporter and study the subcellular localization of scams
为了进一步利用分子生物学方法在基因水平上研究胞外cam的存在，同时研究胞外cam与cam亚型之间的关系，本论文利用绿色荧光蛋白( gfp )作为报告基因研究了大豆cam基因家族( scams )的亚细胞定位。 |
| 9. | It has been demonstrated that kvl . 2 , an isoform of delayed - rectifier potassium channel , is up - regulated in the ischemic neurons . besides , the activity of kvl . 2 is tyrosine phosphorylation dependent , phosphorylation of the tyrosine residue in the amino terminus of the channel by tyrosine kinases suppresses the channel current . therefore , we hypothesize that vegf could enhance the phosphorylation of kvl . 2 , which results in an inhibition of / k
在介导i _ k的钾通道家族中， kv1 . 2是其中的一个亚型，研究报道它在缺血神经元中的表达是增高的；此外，它的活性与其酪氨酸磷酸化水平相关，在其氨基末端的酪氨酸残基经酪氨酸激酶磷酸化后可显著降低其介导的电流的幅度。 |
| 10. | Polymer his - tagged peac1 - gfp efficiently activated myosin mg - atpase activity , which indicated that peacl might take part in correlative living activities in vivo . moreover , this result provided experimental proof in vitro for fusing gfp to actin isoform directly to study the dynamics of microfilaments and its regulation in vivo . we prepared rabbit anti - pea actins polyclonal antibodies using peacl as antigen which being expressed and purified from prokaryotic cells , and the antibodies possessed better immunity activity to pea actins
通过肌动蛋白体外对dnase以及肌球蛋白atpase活性影响的研究，发现单体his - taggedpeac1 - gfp能显著抑制dnase活性，在肌动蛋白聚合条件下能有效激活肌球蛋白atpase活性，这一结果预示着peac1在体内可能参与相关的生命活动，为利用gfp直接与肌动蛋白异型体融合来研究体内微丝的动态变化及其调节提供了实验依据。 |
