Further sequence analysis show that only 6 base pairs of nucleotide and 2 amino acids are different between them . the homological cry3aa gene was expressed in escherichia coli . . and the expressed products which contain a fused peptide of 66 - 97 kilo - dalton was observed by means of sds - page 生物活性测定结果表明该菌株对榆蓝叶甲( pyrrhaltaaenescens ( fairmaire ) )和光肩星天牛等鞘翅目昆虫具有较高的毒力,因此初步确认该菌株属于cry3类; ( 2 )发现该菌株中编码毒蛋白的基因位于质粒上,并且已经成功地克隆到该基因。