A preliminary investigation on the lamin - like proteins in paramecium 草履虫类核纤层蛋白的初步研究
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Lamin - like proteins ; paramecium 类核纤层蛋白草履虫
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Lamin medullares nuclei lentiformis 豆状核髓板
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Lamin medullares thalami 丘脑髓板
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Was first processed by dgd embedment and embedment - free technique and general technique for em morphology . a perinuclear structure consisted of interacted filaments we called lamina - like structure was observed . then using western blot assay , we found a lamin - like component band of 68kd protein in the three - step - fractionated cells . to investigate the distribution of the lamin - like protein in cells , a immunofluorescence experiment for in situ hybridization was designed using goat anti - lamin protein antibodies as the probes . the results revealed that the positive reactivity presented at different part of the cells . the perinuclear cross - actions were distinct , and cross - action with the oral apparatus and the cortex were also obtained 本文以dgd包埋去包埋技术对草履虫的核纤层通过透射电镜和免疫荧光分子杂交等技术进行了观察。结果显示，在核周存在由10nm纤维组成的核纤层免疫荧光结果表明，在核周呈阳性反应，并在其表皮口器等部位呈交叉的阳性反应蛋白分子杂交的反应带在68kd处呈阳性反应。
