Here we managed to make cultured mice peritoneal macrophages be directly influenced by oligochitosan , and be stimulated by ifn - r before oligochitosan added , then measured the changes of gene transcription and translation level of both il - 1 and imf - a , respectively by methods of relatively quantitive rt - pcr and elisa . first , rt - pcr results showed that 18 hours was the most effective time and 40ug / ml was the most effective concentration of oligochitosan , then by the same method , confirm that 4hours is the most effective time and loou / ml is the most effective concentration of ifn - r stimulating . because ifn - r can enhance il - 1 and tnf - a gene expression of macrophages alone , so add ifn - r to microphages alone for 22 hours , then examined by rt - pcr , the results showed that il - 1 and tnf - a gene expression have no remarkable difference compared with the blank contrast group 此外，由于ifn y单独作用也可促进两种细胞因子基因表达，故在巨噬细胞中加入ifn y单独作用22h ，再经阿一pcr检测，发现加ifn y的实验组细胞的几一lp和tnf a基因转录水平与空白对照组相比较无显著性差异，可见，壳寡糖和ifn v对巨噬细胞il lp和tnf一口基因转录水平的影响在作用时间上无一致性，在壳寡糖作用最适时间时，仅受ifn y刺激的巨噬细胞il lp和tnf q基因转录己下降至刺激前水平，因此可以认为， ifn y的加入仅起到对巨噬细胞预刺激使之处于敏感状态的作用，有利于增强壳寡糖对巨噬细胞的作用。
