One of the nuclei at the micropylar pole becomes delimited by a small amount of cytoplasm . 珠孔端有一个核，因为带有少量细胞质而变成界线分明。
2.
About 2s after fertilization in 17 , sperms arrive at micropylar tube ; 3s , sperm penetrates into ovum where cytoplasm is sunken ; 5s , the spermaster come into being ; 20s , fertilization stopper appears ; 60s , sperms in micropylar apparatus begin to disaggregate 精子在受精2s到达精孔管， 3s进入卵子， 5s形成精子星光， 20s形成受精塞， 60s后精孔器内精子开始溶解。有多精入卵现象，极少数卵子多精受精。
3.
The fertilized eggs of hemibnrbus lubco have been observed by sem , tem and om , and main events of fertilization biology have been studied , too . the results suggest : the micropylar apparatus , being composed of vestibule and micropylar tube , is deeply sunken with a short tunnel 对唇(鱼骨)受精过程中发生的主要事件进行组织切片、透射和扫描电子显微镜观察，结果表明：精孔器属深凹陷、短孔径型，精孔管一次只能通过一个精子。
4.
Morphology all ephedra species from china are illustrated with great details on male and female cones . the photographs provide valuable information of characters including peduncle of cone , bract , and micropylar tube etc . for classification and identification of ephedra species from china 2 、形态学提供了中国麻黄属种类的标本(包括模式标本)照片资料及中国麻黄属植物的雌球花和雄球花的照片资料，为麻黄属分类和系统学研究提供了证据。
5.
( 4 ) fluorescent investigation shows that pollen started to germinate about 20 hours later after deposited on the stigmas , only 1 or 2 pollen tubes can penetrate ovary to micropylar end on the fifth day . callose plugs evenly formed in the pollen tube , and sometimes callus deposited randomly along pollen tube wall and even the whole tube wall . ( 5 ) 16 species of insects visited c . praecox in nanjing , belonging to hymenoptera , diptera , coleoptera , thysanoptera and spiders ( arachnoidea ) ; apis cerana cerana fabricius , aldrichina grahami ( aldrich ) , epistrophe balteata de gee , musca hervei villeneuve are its main efficient pollinators ( 5 )南京地区蜡梅的访问昆虫约16种，分属膜翅目hymenoptera 、双翅目diptera 、鞘翅目coleoptera ，缨翅目thysanoptera 、及蛛形纲arachnoidea ，主要传粉昆虫有4种：膜翅目蜜蜂科东方蜜蜂中华亚种apisceranaceranafabricius 、双翅目蝇科muscidae黑边家蝇muscaherveivillcneuve 、丽蝇科calliphoridae巨尾阿丽蝇aldrichinagrahami ( aldrich )及食蚜蝇科syrphidae黑带食蚜蝇epistrophebalteatadegeer ；亮叶蜡梅的访问昆虫约18种，分属鞘翅目coleoptera 、半翅目hemiptera 、缨翅目thysanoptera 、膜翅目hymenoptera 、双翅目diptera 、鳞翅目lepidoptera ，另外有蛛形纲arachnoidea的蜡橄ch俪ononthuspr口ecox ( l
6.
Cortical reaction comprises latent period , developmental period , climactic period and declining period . about 35s after fertilization , a few cortical alveoli begin to discharge from the low - latitudes of the animal hemisphere , later , the same pross happens in the near of micropylar apparatus , and then cortical reaction extends to the animal hemisphere and the vegetal hemisphere 皮层反应可分为潜伏期、发展期、高潮期、衰退期， 20s开始于动物极低纬度区，稍后出现在精孔器前庭附近，随后在这两个始发区向四周扩散。
7.
It was also found that the nuclear envelope disappeared earlier at the micropylar end than that at the chalazal end . the speed of the programmed cell death were different in three nonfunctional megaspores at the micropylar end and their relationships with the neighboring cells were also different . the biological significance was discussed about these phenomenon 还发现了大孢子母细胞合点端的核膜先于珠孔端的消失，并且珠孔端3个大孢子的死亡时间不同，与相邻细胞的关系也不同，进而讨论了这些现象的生物学意义。
