| 1. | Effects of electroacupuncture on the expression of nestin in neural stem cell after focal cerebral ischemia in rats
电针对局灶性脑缺血大鼠神经干细胞巢蛋白表达的影响 |
| 2. | 3 . to probe into the differentiation route of mscs to neurocyte , the specific mark of nerve stem cell , nestin , was detected by rt - pcr
为了探索msc向神经细胞分化过程中的路径，本研究利用rt - pcr技术检测了诱导前后的细胞对神经干细胞特异性标志nestin的表达。 |
| 3. | After 4 years hard work , their group has finally cloned mouse nestin gene , an intermediate filament protein expressed specifically in neural precursor cell during cns development
从事发育神经生物学及分子神经生物学研究，主要研究方向为脑发育和脑功能的分子和细胞机制。 |
| 4. | The result showed that mscs did not express nestin , and induced cells expressed nestin . it suggested that mscs differentiated firstly into nerve stem cells , and then differentiated into neurocyte
结果发现诱导前的msc不表达nestin ，诱导5h后的细胞表达nestin ，表明msc经神经干细胞向神经细胞分化。 |
| 5. | Continuous passage of neurospheres was used to isolate , culture nscs from dentate gyrus of hippocampus of newborn rats and to observe the self - renewal of neurospheres , which were immunostained with nestin
采用神经球连续形成法从新生大鼠海马齿状回分离、培养神经干细胞，通过连续传代培养观察其自我更新能力。 |
| 6. | But the understanding of effects of gm - csf on nscs is still poor . experimental precedure 1 immunohistochemistry was applied to detect nestin - positive cells in dentate gyrus of hippocampus of rats at the first day after birth
状回神经卜细胞鉴走、分化及6翎比砍、乃的农达方法1本实验采用免疫组化技术观察出生id的大鼠海马齿状回nostin表达。 |
| 7. | Results : the neural stem cells isolated in newborn rats had the potential of forming clones and serial passage , expressing neuroepithelial stem cell protein ( nestin ) and differentiating into neurons and astrocytes and oligodendrocytes
结果：新生大鼠脑组织分离的细胞具有连续传代和增殖能力，表达神经上皮干细胞蛋白，诱导分化后的细胞表达神经元细胞、星形胶质细胞、少突胶质细胞特异性蛋白。 |
| 8. | Two days later ( that is , these cells had been cultured for ten days in vitro from isolation ) these cells were collected and immunocytochemistry was performed by anti - nestin to identify nscs and by anti - brdu to evaluate nscs " proliferating capability
3 . 4ap25一35和ap25一35 +雌激素作用后细胞增殖能力检测对照组神经干细胞brdu阳性细胞率约为50 % ;雌激素保护组及ap毒性组brdu几乎未见阳性细胞。 |
| 9. | But they give expression to nestin and receptors of epidermal growth factor ( egf ) and basic fibroblast growth factor ( bfgf ) ; secondly , they possess the capacity for self - renewal ; thirdly , they possess the capacity for mul tipotential ity , namely differentiating into various cells in the central nervous system
同时表达神经干细胞标志性蛋白-神经巢蛋白( nestin ) ； ( 2 )自我更新能力； ( 3 )多潜能性，即能分化成神经系统的各种细胞。 |
| 10. | We detected the transcription and translation change of different thrs genes by semiquantitative reverse transcription - polymerse chain reaction ( rt - pcr ) analysisjelisa respectively . at same time the differentiated cell were identified by immunocytochemistry method . results ( l ) nsc induced by mitogens were nestin - positive and incorporated by brdu
以pdgf 、 t _ 3对nsc进行诱导分化，并分别用rt - pcr半定量法、 elisa法检测nsc在不同诱导因子作用下分化前后thr各亚型在转录与翻译水平上的表达变化，用免疫细胞化学方法鉴定分化后的细胞类型。 |
