In the early neurula , cortactin staining signals were detected in the developing primodium of forebrain as early as 10 . 5 hpf ( hours post fertilization ) , at the same time the tailbud areas were highly stained too . later , the staining was concentrating in the developing neural rod while paraxial mesoderm derivatives were not stained 在早期神经胚，神经系统中的前脑原基最先出现染色，并保持头尾两端处染色最强的图式，随后，染色集中分布在发育中的神经龙骨中，但近轴中胚层细胞没有染色。
2.
Whole mount in situ hybridization showed that amphihmgb transcripts were present ubiquitously in the early embryos from zygote to neurula , but they decreased gradually in mesoderm and endoderm and neural tube with development , and became restricted to epidermal cells in the hatched larva . it appear s that hmgb plays a role in the mitosis and differentiation of embryonic cells . another amphioxus cdna , amphiubf80 , encoding ubiquitin / ribosomal fused protein s27a , was isolated from the gut cdna library of b . belcheri tsingtauense S004cdna序列编码一个156个氨基酸残基组成的蛋白质，与人的泛素蛋白ubf80具有较高的氨基酸序列同源性( 145 / 156同源) ，且该蛋白具有泛素蛋白ubf80所共有的两个结构域，即由72个氨基酸组成的泛素介导的蛋白降解结构域和由48个氨基酸组成的核糖体蛋白s27a结构域。
