Sun 7 freiburg vs carl zeiss jena sun 8 greuther furth vs paderborn 星期日8荷甲飞燕诺对格罗宁根
2.
And another nine strains and strains hclv , shimen of two major reference strains of china belonged to group i . in addition , strain p97 was the special individual G皿z 、 gxnn 、与属1群的中国主要参考毒株hclv 、 shimen在遗传关系上较远，与italy株和paderborn株比较接近。
3.
But compared the e2 genes of strains gxnn , gxlz with paderborn strain , the homology of nucleotide were 95 . 5 % and 95 . 6 % ; and that of amino acid were 96 . 7 % , 97 . 5 % respectively 但广西的这两株流行毒株与德国的paderborn和意大利的italy株的核苷酸和氨基酸的同源性都比较高。 gxnn 、 gxlz与paderborn株的核苷酸同源性为95 . 5 、 95 . 6 。
4.
Phylogenetic tree analysis based on the genomic nucleotide sequence showed that the 14 csfv stains were divided into two groups . gxwz02 , paderborn and 39 were classified into group 1 , and other strains were classified into group ii . and the genetic relationship is very close between gxwz02 and paderborn 系统发育树分析表明，所比较的14株csfv被分为两个群， gxwz02 、 paderborn和39这3个毒株被归为群，其余的11个毒株被归为群， gxwz02与paderborn的亲缘关系最接近。
5.
According to the published nucleotide sequences of genome of classical swine fever virus ( csfv ) strains shimen , hclv and paderborn , 11 pairs of specific primers were designed and synthesized , eleven fragments had been successfully amplified from csfv gxwz02 strain by rt - pcr . these amplified fragments were cloned into pgem - t or pmd18 - t and sequenced 根据已发表的猪瘟病毒( classicalswinefevervirus ， csfv ) shimen株、 hclv株和paderborn株的全基因组序列，设计并合成11对引物，应用rt - pcr技术，成功地分11个片段扩增了csfv广西流行毒株gxwz02的全基因组，并将这11个基因片段克隆到pgem - t和pmd18 - t载体上，测定其核苷酸序列。
