Pasteurellosis denotes an infectious disease in which the microorgunisms pasteurella multocida or pasteurella hemolytyca are causally involved . 巴氏杆菌病是病原涉及多杀性巴氏杆菌或溶血性巴氏杆菌的一种传染病。
2.
Diagnostic techniques for swine pasteurellosis 猪巴氏杆菌病诊断技术
3.
A dot - ppa - elisa method has been developed to detect the antibody in swine serum against pasteurellosis . the capsule polysaccharide and outer membrane protein of pasteurella multocida was prepared as the diaphragm antigen 本研究提取猪巴氏杆菌菌株的荚膜和外膜蛋白抗原，首次建立了检测猪巴氏杆菌病血清抗体的dot - ppa - elisa方法。
4.
Experiment shows that the antigen extracted by autoclave is best for dot - ppa - elisa . applying the x2 test , optimal concentration of antigen was determined , and the optimum dilution of enzyme hrp - spa was 1 : 10 . the high specificity of dot - ppa - elisa was proved by the specific blocking test , and also by the cross - reaction test in which the diaphragm does not react with the antibodies against salmonellosis , pasteurellosis , chlamydiosis , hcv , ppv , brucellosis , erysipelas suis , colibacillosis , prv 试验中对多种方法提取的诊断抗原进行了比较、筛选；确定了dot - ppa - elisa的最佳工作条件；测定了st - 171株的免疫猪血清抗体及c群、 2型人工感染猪血清抗体效价，并初步确定了该方法的阳性标准。
5.
The high specificity of dot - ppa - elisa was proved by the specific blocking test , and also by the cross - reaction test in which the diaphragm did n ' t react with the antibodies against pasteurellosis , streptococcosis , colibacillosis , chlamydiosis , hcv , ppv , brucellosis , prv and foot - mouth disease . the diaphragm has good sensitivity and could detect some salmonella - positive test serum which has been diluted to 1 : 2048 . stored at 4 for at least 6 months or at 10 - 25 " c for 4 months , the sensitivity and specif icity of the diaphragm did n ' t change , so it has good stability 本研究制备的诊断膜片特异性强：不与猪衣原体病、猪口蹄疫病、猪大肠杆菌病、猪布氏杆菌病、猪瘟、猪伪狂犬病、猪细小病毒病、猪巴氏杆菌病、猪链球菌病的阳性血清发生交叉反应；诊断膜片具有良好的敏感性，能够检测到1 ： 2048稀释的动物试验阳性血清；膜片的保存期长，在10 25可保存4个月、 4条件下至少可保存6个月其灵敏度不变。
