| 1. | After that we determined the presence of angiostatin gene in the putative recombinant virus with pcr analysis
之后利用pcr分析是否获得重组angiostatin的杆状病毒。 |
| 2. | The conserved cystein residues also present in the cub domain of clsp at position of 36 , 94 and 112
Rt一pcr分析结果证实clspmrna在dc中存在一种时间依赖性的表达模式。 |
| 3. | In pbivpt , it is under control of camv35s promoter . in pevp7 , it is under control of tomato - fruit - specific tfp promoter
Pcr分析结果为阳性的植株有17株(转pbivp7抗性植株10株，转pevp7抗性植株7株) 。 |
| 4. | Pcr ( polymerase chain reaction ) , southern blot analysis performed toward 10 transgenic rice plants revealed that foreign genes were integrated into the genome of these plants
选取部分植株进行pcr分析、 dna点杂交、 pcr southern杂交。以上分子检测结果表明，绝大部分经抗性筛选得到的转化植株的基因组中已整合有外源基因。 |
| 5. | The results showed that ybt - 803 contained cryia ( a ) , cryia ( b ) and cryia ( c ) genes , and ybt - 791 contained cryla ( b ) , cryla 拢 } and cryll genes . these genes were located in > 38mda plasmids
利用cry a ( a ) ecor - f片段rna探针和cry混合特异引物对本室筛选的苏云金芽胞杆菌菌株ybt803和ybt - 791的质粒进行了基因杂交定位和基因型的pcr分析。 |
| 6. | Armigera was analysed and then registered in genbank and accession number was af515667 . the cdna encodes a protein with a plltative 2l amino - acid signal peptide and a 425 amino - acid mature protein with molecular mass of 50 kda
连接产物转化jm ，提取质粒进行酶切及pcr分析，将此连接产物命名为pchto将pcht转化blzi （ de3 ） ，经iptg诱导，提取菌体内容物sds page电泳。 |
| 7. | The annfaf cdna with camv35s promoter and nos terminal formed the antisense fusion gene . the engineered stain eha105 ( prokii - annfaf ) was constructed by conducting the prok ii - annfaf to a . tumefaciem eha1 05 and was used to transform strawbern " . the position clones were obtained via using selection marker and pcr anah sis
采用直接导入法将其导入了具有超强致病力的根癌农杆菌eha105中构建了用于植物遗传转化的工程菌株eha ( prok - annfaf )并转化草莓“新世纪1号” ，通过kan筛选pcr分析获得了阳性克隆。 |
| 8. | The genomics dna of the transformants was extracted and assayed by pcr with nptii primer camv35 / cp primer and the results indicated that the chloroplast shsp gene has been integrated into the genomics of the tomato . then the transgenic tomato were exposed to low temperature ( in winter , on natural condition , the top temperature was 15 ? and the lowest temperature was 5 and a set of physiology parameters were measured after 6 weeks . the results were shown as follows : 1 ) effect on growth height of the transgenic tomato and the control plants after 6 weeks at low temperature showed that the transformants had been grown faster than the control . in addition , the leaves of the control plants appeared to be much reder than the transgenic tomato , and the change were obvious followed by far from the treated time at low temperature , which suggested that the constituently expression of the chloroplast shsp had some protective fountions to the tomato at low temperature
提取转基因番茄基因组dna ，分别以npt和35s cp引物对其进行pcr分析，结果表明叶绿体shsp基因已整合进番茄基因组中；对转基因番茄进行低温处理(冬季，自然条件下(无加热的温室) ，白天最高温度15 ，夜间最低温度5 ) ，生长6周后，检测转基因番茄的系列生理指标，主要结果如下： 1 )生长势：测量转基因番茄与对照(未转基因番茄)的株高，结果显示转基因植株生长明显快于对照，且从外观上看到对照叶片发红程度远大于转基因植株，随着低温时间延长，对比更加明显，说明叶绿体shsp的组成性表达在低温下对番茄具有一定的保护作用。 |
