| 1. | Modified brazzein gene was amplified successfully from the wild brazzein gene by pcr 通过pcr扩增,获得突变的brazzein基因。 |
| 2. | When plants were grown , dna were extracted and transgenic plants were identified by pcr amplifying 植株成年后,取叶片提取基因组dna ,进行pcr扩增。 |
| 3. | 3 . establishing contamination free pcr amplification system by adding ung enzyme . 4 于反应体系中引入ung去污染酶,建立了无污染荧光pcr扩增体系; 3 |
| 4. | 1 vertor , dh5a competent cells and long template expanded pcr kit were from invitrogen company 1载体, dh5a感受态细菌及pcr扩增试剂购自invitrogen公司。 |
| 5. | 6 . in this study , establishing contamination free pcr amplification system by adding ung enzyme 6 、于反应体系中引入ung酶,建立了无污染的荧光pcr扩增体系。 |
| 6. | The transformation of hairy roots was confirmed by pcr amplification of rolb and rolc genes of ri plasmid from a . rhizogenes Pcr扩增结果证实, ri质粒的t - dna部分已在红龙草毛状根的基因组中整合及表达。 |
| 7. | Results 1 pcr amplification result 18 among 48 isolates were amplified successfully and positive rate was 37 . 5 % 实验结果一jcr扩增结果对48株低传代临床分离株进行hcmvul136全序列pcr扩增,结果18株阳性,阳性率37 |
| 8. | The third part of this study is analysis of 16s - 23s rdna its . specific primers were used and dna segments of 500bp and part sequence were obtained J区研究,采用特异引物进行pcr扩增,得到长度约为500hp的片段,并获得部分序列。 |
| 9. | The beth gene was predicted to encode a 55 . 2 kda protein ( 504 amino acid residues ) with 12 transmembranes regions 通过反向pcr扩增获得beth基因片段的侧翼序列。通过blast比较,证实获得beth基因的全部编码序列。 beth属于bcct家族。 |
| 10. | 042bm noea was obtained by pcr , it is identical to that of strain 1021 at 99 % level , and similarity of their noea is 97 % 通过pcr扩增,获得了042bm的noea基因。该基因与苜蓿中华根瘤菌1021noea的同源性为99 ,而与noea的相似性为97 。 |