| 1. | Pfge of shigella flexneri 4c isolates from food - poisoning outbreaks and sporadic diarrhea patients
型暴发和散发菌株的脉冲场凝胶电泳分型 |
| 2. | Pfge analysis of 6 ecor ] digested bacs clones which were derived from the library and contained lmbr1 sequence proved the overlap among the bacs
对筛选得到的6个含lmbr1序列的bac克隆酶切分析的结果表明bac克隆之间是相互重叠的。 |
| 3. | All the five e . coli o157 : h7 strains isolated from patients with diarrhea and / or hus ( hemolytic uremic syndrome ) in 1999 are shiga - toxin ( stx ) gene positive and have the same pfge pattern
研究发现， 1999年在江苏省徐州市从腹泻病患者、 hus患者分离的5株产生志贺毒素的大肠杆菌0157 ： h7菌株，属于一个pfge型。 |
| 4. | The dnd phenotype was also found in dna of mycobacterium smegmatis mc2155 by pfge . query of the amino acid sequence of dnd cluster from s . lividance to the uncompleted genome sequence of me2155 , a homologous gene with a 38 % identity to dnda was found and was tentatively named mddl
将变铅青链霉菌的dnd基因簇与mc ~ 2155的基因组序列(未测完)进行同源比较后，发现mc ~ 2155中存在dnda的同源基因(暂命名为mddl ) ，且二者在氨基酸水平上的同一性为38 。 |
| 5. | Pfge analysis of these 21 blocked strains revealed a common chromosomal deletion in the 300kb asel fragment which might be responsible for antibiotic 5102 - iii biosynthesis . so the 300kb fragment was recovered and used as probe to hybridize with 10 - 22 genomic library . cosmids in this region were aligned and suitable fragments in this region were selected and used further for the construction of gene replacement plasmids
以此片段为探针钓出了位于该区域的文库克隆，并利用指纹印迹和杂交技术将这些文库克隆排列起来，进而以位于该区域的不同位置的片段做臂构建了用于转化和接合转移用的基因置换质粒，并试图通过基因置换将该区域置换下来，但尚未得到最终结果。 |
| 6. | We found that sit variant gene ( slt2vha ) was identified in strains e . coli o157 : h7 isolated from patients and dung beetles 2000 in xuzhou city , jiangsu province . the primers used for stx2 variant analysis are shown in tablel . genomic dna restriction fragments digested by pstiwere sonthern - blotted and hybridized with an stx2 - specific dna probe . the probe was prepared fromed a 285 - bp pcr productof the strain 882364 stx2 gene obtained by using the specific primer pair ( tablel )
2000年在江苏省徐州市铜山县腹泻病患者的粪便标本分离的10株产生志贺毒素的菌株以及从蜣螂肠道分离到的4株产生志贺毒素的大肠杆菌o157 ： h7 ，属于另两个pfge型，和1986 、 1987 、 1988年在徐州市腹泻病患者的粪便标本分离的菌株pfge图谱不同。 |
| 7. | In order to understand the epidemiological behaviors of this pathogen , we studied strains e . coli 0157 isolated from patients and dung beetles in 1999 and 2000 in xuzhou city , jiangsu province , by methods of pcr , antimicrobial susceptibility , biochemical reaction and pfge ( pulse - field gel electrophoresis ) of chromosomal dna digested by restriction enzyme xbal
为了探索我国大肠杆菌o157 ： h7感染的流行病学特点，我们使用了聚合酶链反应、生化反应、抗生素敏感实验、脉冲电场凝胶电泳等分子生物学方法，对我国1999 - 2000年徐州地区分离到的部分大肠杆菌o157 ： h7进行了分析。 |
