Photoreactivation systems have been found in many organisms from bacteria to marsupials . 在从细菌到有袋类动物的许许多多有机体中,已经发现光致反应系统。
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5 . after induced with iptg , jm109 with pgex - 4t - l / 6 - 4 was radiated by uv for 30 seconds , cultivate these e . coli with light or without light . the survival rate proved the gene of d . salina ( 6 - 4 ) photolyase has photoreactivation 5 .工ptg诱导过的转化菌,经过30秒的紫外光照射后,分别进行光培养或暗培养,计算存活率,从而证明了及sa了z ’ na的( 6一4 )光裂合酶在大肠杆菌中可进行光修复活性作用。
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Many organisms produce enzymes , termed photolyases , that specifically bind to these damage products and split the via a uv - a / blue light - dependent mechanism ( photoreactivation ) , thereby reversing the damage . these two photolyase are specific for either cpds ( cpd photolyase ) or 6 - 4 products ( 6 - 4 photolyase ) . a gene that expresses a protein with 6 - 4 photolyase activity in vitro , was recently cloned from high organisms ( arabidopsis thaliam , drosophila melanogaster , danio rerio , xenopus laevis and homo sapiens ) 目前已从高等生物拟南芥、鲐类、果蝇、人类和非洲爪蟾蜍属中克隆到有( 6 - 4 )光裂合酶活性的基因,本研究从盐生杜氏藻dunaliellasalina中克隆到( 6 - 4 )光裂合酶的基因,并将该基因在大肠杆菌中得以表达,这是首次在藻类中克隆到( 6 - 4 )光裂合酶基因,对光裂合酶的研究具有重要意义。