| 1. | Determination of iodine and trace element in different parts of laminaria japonica and underia pinnatifida 海带和裙带菜碘及微量元素含量的测定 |
| 2. | The extracting technology of fucoidan from sporophyll powder of sea mustard undaria pinnatifida suringar by enzymatic hydrolysis 热水浸提法提取裙带菜孢子叶粉岩藻聚糖硫酸酯的工艺研究 |
| 3. | The separating technique of the light active pigment - protein complexes from brown algae undaria pinnatifida was developped in this paper 以褐藻裙带菜( undariapinnatifida )为实验材料,建立一套能够分离出有光活性色素蛋白复合物的技术路线。 |
| 4. | The results show that c . pinnatifida bunge has the strength antioxidative effect , and the antioxidative effect increased with increasing concentration 用流动注射化学发光法测定了山楂果的抗氧化性能,实验结果表明:山楂果具有较强的抗氧化性能,并且抗氧化性能随着浓度的增加而增加。 |
| 5. | The ultrasonic method was used for extracting flavonoids of c . pinnatifida bunge and the flavonoids were determined by spectrophotometry , the recovery is 96 % ~ 105 % and the coefficient of variation is 0 . 14 % 摘要采用超声波法从山楂果中提取黄酮类化合物,并建立了山楂果中黄酮化合物的光谱分析方法。 |
| 6. | A genetic transformation model for the brown seaweed undaria pinnatifida has been primarily set up by using micro - particle bombardment as the method , female or male gametophytes as the gene recipients , hybridization as the regeneration route and chloramphenicol , hygromycin or basta as selective reagents 本文从转化受体、转化方法、报告基因、再生途径、筛选方法等方面对裙带菜的遗传转化进行了研究。首先,分离并建立了裙带菜雌雄配子体的无性繁殖系,进行了裙带菜的不同再生途径的研究。 |
| 7. | By using primers designated for lacz gene , pcr result suggested an integration of lacz into u . pinnatifida genome . nine reagents including penicillin g , kanamycin , g418 , teicoplanin , zeocin , chloramphinicol , hygromycin , basta and methotrexate were tested as selective reagents for selection of transgenic sporophytes . the results showed that young sporophytes of u . pinnatifida were sensitive to chloramphinicol and hygromycin and very sensitive to basta which suggest the potential of using the resistance genes as selectable markers ,为了解决转基因裙带菜的筛选问题,进行了裙带菜幼孢子体对不同筛选压力的敏感性实验,包括抗生素类的青霉素g 、卡那霉素、 g418 、硫酸链霉素、盐酸洁霉素、 zeocin 、氯霉素、潮霉素,除草剂类的basta ,氨甲喋呤类的氨甲喋呤,结果显示裙带菜幼孢子体对氯霉素、潮霉素敏感,对basta非常敏感。 |
| 8. | Several vegetative clones of male or female gametophytes of u . pinnatifida has been induced and parthenogenesis of female gametophytes has been observed . 90 % of the gametophytes can grow into young sporophytes one month after fertilization . no staining background of either lacz or gus was found in different tissues of u . pinnatifida 进行裙带菜配子体孤雌生殖、孤雄生殖和雌雄受精的再生途径实验,结果没有得到孤雄生殖的裙带菜,孤雌生殖的效率低、时间长且发育不正常,雌雄受精的再生途径一个月左右90 %以上的雌配子体都正常萌发成幼孢子体。 |