Proteomic analysis of 042bma - km was carried out using two - dimensional gel electrophoresis . deletion of noea had a pleiotropic effect on protein synthesis levels 对042bma - km进行的蛋白质组初步分析发现， noea基因的敲除对蛋白质合成水平具有多效性影响。
2.
The two genes were subcloned into pet30 vector , and recombinant proteins with predicted molecular weight were achieved . in this paper , we constructed the recombinant e . coli highly expressing pil - 10 in order to study the pleiotropic immunological regulations in various diseases 然后将p35基因和p40基因分别亚克隆到原核表达载体pet30c 、 pet30b中，用iptg诱导培养，分别表达出了预期分子量大小的重组蛋白。
3.
Interferon - is an antiviral cytokine produced by activated t cell and nk cell . it upregulated mhc ii antigen expression , influenced the cellular and humoral immune response and has pleiotropic regulatory effects on immune system . one gene was amplified by reverse transcription - polymerase chain reaction ( rt - pcr ) in splenocytes which was stimulated with cona for 24 hours -干扰素( interferon - ， ifn - )主要由活化的t细胞及nk细胞产生，具有抗病毒作用，也具有上调mhc ( majorhistocompatibilitycomplex , mhc )类抗原分子的表达等作用，是影响机体细胞免疫和体液免疫反应的一类具有多种调节效应的细胞因子。
4.
Lymphotoxin ( lt ) is a kind of pleiotropic lymphocyte - secreted cytokine which mediates a large variety of inflammatory , immunostimulatory , and antiviral responses . in order to increase the antitumor activity of lymphotoxin and reduce its side effects , the recombinant plasmid pet36b - lt 27 was constructed to express soluble fusion protein cbd - lt 27 . the active form of lt 27 could be collected directly with several simple steps by three kind of components on the expressed fusion protein 本研究通过构建表达n端缺失27个氨基酸的淋巴毒素融合蛋白的重组质粒，在大肠杆菌中实现融合蛋白的可溶及分泌表达，同时利用表达载体上的几种特殊序列经简单的分离纯化步骤直接获得大量的有生物活性的淋巴毒素缺失体lt 27 ，为寻找一种高抗肿瘤活性、低临床毒副作用的生物抗癌药物进行了有效的探索。
5.
S . lividans mutant strains zx1 ( dnd cluster deleted ) and zx64 ( dnda disrupted ) had pleiotropic mutations including low mel expression and poor sporulation . it was speculated that dnda together with its downstream dna ( 2 . 5kb ) might be involved in these two phenotypes because dnda together with its downstream dna could restore normal sporulation and mel expression to zx64 , while dndb and dndc had no such effect because lai and la2 showed no obvious difference in these two phenotypes from wild type s . lividans 1326 另外通过比较这几个突变株及野生型菌株在产孢和黑色素基因( mel )表达方面的差异，推测dnda及其下游区域与变铅青链霉菌的产孢和刺激外源黑色素基因的表达有关，而dndb和dndc则与之无关，因为la1和la2在这两种表型上与野生型菌株无明显差异。
6.
It possess high homology with those of ck2 ( casein kinase 2 ) a subunits from various species . it is now well established that protein kinase ck2 is a pleiotropic and abiquitous serine or threonine kinase , which is highly conserved during evolution . we obtained the complete cdna sequence of ck2 a via 5 " race 酪蛋白激酶ck2是目前发现的普遍存在于真核生物中的丝氨酸苏氨酸型蛋白激酶，进化上具有高度的保守性并参与了多种生理功能的调控，因而克隆它的基因具有重要意义。
