Multi - locus dna fingerprint technique was used to check the chimerism of chimeric mouse generated by injecting es cells into blastocysts and to detect whether the chimeric mouse is a germ - line chimeras . the results indicated that : the multi - locus dna fingerprint with a new synthesized probe - jl - 02 , has enough polymerism and good stability , and should be very useful to monitor the chimerism in different tissues of es cell chimeric mouse and to check whether an es cell line has the capacity to enter the germ line , especially when involving strains that can not be discerned with coat color or biochemical markers 尝试应用多位点dna指纹技术，检测经过胚胎干细胞es细胞途径所获得的嵌合体小鼠中es细胞在各种脏器中的嵌合情况检测es细胞在嵌合体小鼠中是否实现种系传递。结果表明：采用新型的人工合成的寡核苷酸多聚体探针jl - 02探针的多位点dna指纹图谱，具有足够的多态性和很好的稳定性。
