| 1. | This comparison uses the following model of procaryotic transcription .
下面以原核细胞转录作用为模式进行比较。 |
| 2. | Procaryotic , expression of human heat shock protein 70 gene
人热休克蛋白70基因的原核表达 |
| 3. | Through these experiments we realized cloning of the gene of rbp and its expression in procaryotic cell
Colidh5中获得了有效表达，为以后的研究工作奠定了基础。 |
| 4. | The object of this study is halr , focus on its molecular cloning , construction of eucaryote expression vector and procaryotic expression
本课题选用halr作为研究对象，重点研究其分子克隆、真核表达载体构建及原核表达。 |
| 5. | Target gene was cloned into the procaryotic fusion expression vector pet28a ( + ) , then subcloned into the eucaryote expression vector pcdna3 . 1 ( + ) after sequence analysis
将halr基因克隆到原核融合表达载体pet28a ( + )上，序列分析后亚克隆到真核表达载体pcdan3 . 1 ( + )上。 |
| 6. | We integrated dh gene into procaryotic expression vector for the first time , which establish foundation for obtaining dh recombinant protein to research the structure and function of dh
本研究首次将dh基因整合入原核表达载体中表达，为大量获取dh重组蛋白，研究dh的结构及功能奠定了基础。 |
| 7. | Procaryotic expression vector recombinant was transformed into competent bl21 , induced and expressed by iptg revulsant , explored the best inducing time and the best concentration of revulsant
将原核表达载体的重组质粒转化大肠杆菌bl21 ( de3 ) ，用iptg诱导物进行诱导表达，探索最佳诱导时间和诱导物的使用浓度。 |
| 8. | The coding region of cdna was cloned into procaryotic expression vector pet30a and overexpressed in e . coli bl21 ( de3 ) . the cyclase proteins extracted from bacterial culture were found largely in the insoluble protein fraction
Cdna编码区序列被克隆进原核表达载体pet - 30a ，并在大肠杆菌bl21 ( de3 )中诱导表达，但过量表达的蛋白主要是以不溶性蛋白形式存在。 |
| 9. | These results suggested that axud1 protein functioned in a different way in the expression of cell cycle and apoptosis related proteins induced by tgf - 1 in tumor cells . 5 . axudl cdna fragment was cloned into procaryotic expression vector pqe30 and t
5 . axudlcdna片段成功地克隆入原核表达载体pqe30 ，并用iptg诱导出axudi融合蛋白的表达，这为进一步纯化axudi蛋白，研究axudi蛋白的免疫组织化学定位和结构功能奠定了基础。 |
| 10. | On the bases of designing a primer pair , we obtained the coding domain sequence of rbp by pcr from cdna library . then the gene was cloned into pgem - t vector , the dna sequencing showed that the cloned gene was in agreement with the reported sequence . then the tageted gene of rbp was further subcloned into a procaryotic expression vector pbv220 and constructed recombinant plasmids was named pbv220 - rbp . in order to expresse rbp in procaryotic cell efficiently , the recombinant plasmids was introduced into e . colidhs a straints . expression of rbp was induced by temperature induction
本实验在合成该蛋白基因上下游引物的基础上，利用pcr技术，从人睾丸cdna库中钓取目的基因，并克隆于pgem - t载体中，经序列测定证明与文献报道基本一致，再将目的基因从重组克隆质粒中亚克隆于pbv220原核表达载体中，转化宿主菌，经温度诱导后，进行sds - page电泳分析，发现在约21kd位置上出现了一条明显的蛋白带，与预期相符。 |
