| 1. | These proteins exhibited various cellular functions , most of them have not been reported to be relevant to radioresistance
这些蛋白行使细胞的各种功能，绝大部分未曾报道与辐射抗性有关。 |
| 2. | The results of present paper suggest that it is feasible to discovery novel radioresistance - associated proteins by proteomics methods
这些结果说明利用蛋白质组学的方法来发现耐辐射球菌辐射抗性相关蛋白是可行的。 |
| 3. | Thus clarifying the functions of these proteins may help us to better understanding the mechanisms of extreme radioresistance in this organism
因此，解释这些功能未知的蛋白可能会帮助我们更好的理解耐辐射球菌极端辐射抗性机理。 |
| 4. | Though it has been researched for almost fifty years since discoveried in 1956 , little has been known about the involvement of dna damage response pathways accounting for the radioresistance in this organism
虽然对它的特殊抗性机制有近50年的研究历史，但对于其中的分子机制仍知之甚少。 |
| 5. | In order to reveal the mechanisms of extreme radioresistance and dna repair in deinococcus radiodurans , we examined proteome changes in a wild type strain following y - irradiation using 2 - dimensional polyacrylamide gel electrophoresis and silver staining
为了研究耐辐射球菌极强的辐射抗性与dna修复机理，我们应用双向电泳结合银染的方法考察了野生型菌株kd8301在射线照射前后细胞体内总蛋白组的变化情况。 |
| 6. | When compared with kds301 ( lexa + ) , xe ( lexa - ) cells showed quite similar levels of survival curve to y - irradiation and mmc , indicating lexa gene had no effect on radiation resistance of deinococcus radiodurans . deinococcus radiodurans " lexa had no revelance with its extremely radioresistance . the recombinant plasmid pza172 was constructed by cloning the lexa gene of deinococcus radiodurans into the vector plasmid puc19 in the downstream of lacz promoter
对lexa基因突变体xe的研究表明，其辐射抗性与lexa基因野生型的抗辐射菌kd8301相近，存活曲线上均显示有一个宽大的肩区，结果发现lexa基因的突变并没有改变抗辐射菌的独特抗性，说明lexa基因与辐射抗性无关。 |
| 7. | Because there are no enough efficiency methods to dispose pollute , so to study and develop new methods is very urgent . we do some work to study on the radioresistance bacteria deinococcus radiodurans to construct one super bacteria , which could resolve the pollution in a complex environment contaminated by both radiation and organic compound
同时针对耐辐射奇球菌deinococcusradiodurans对电离辐射、紫外线、干燥、强氧化剂和一些化学诱变剂的惊人的抗性，对构建在放射性和有机卤代物污染的复合环境中具有生物修复功能的超级基因工程菌方面做了初步的研究，以发展有效清除环境污染的新途径。 |
| 8. | Recently , a new gene dr0167 ( pprl or irre ) that serves as a general switch for downstream dna repair and protection pathways via its regulatory function on the gene expression of reca , ppra was discoveried . expression of d . radiodurans pprl also promotes dna repair and protection pathways and enhances the radioresistance of e . coli . this finding provides a new clue to understand the mechanism of dna repair , especially double strand break ( dsb ) repair
最近我们实验室在耐辐射球菌电离辐射敏感株中鉴定了一个与电离辐射抗性相关的基因ppri ,该基因可能通过调控dr细菌reca 、 ppra等基因的表达加速对电离辐射引起的dna损伤修复，而在大肠杆菌中表达ppri基因能促进reca 、 soda等表达水平显著提高，使其抗辐射和抗氧化能力明显增强，这将为我们理解其特殊抗性机制，特别是双链断裂修复提供新的线索。 |
