| 1. | Two recombinant viruses : vac - ha2 - gfp and delac9 - ac - ha2 - gfp was expected to give the answer
构建了表达haz与acg两个同源基因的重组病毒vac - haz一g印。 |
| 2. | After that we determined the presence of angiostatin gene in the putative recombinant virus with pcr analysis
之后利用pcr分析是否获得重组angiostatin的杆状病毒。 |
| 3. | The newly molted 5th instar larva of silkworm bombyx mori was infected with the recombinant virus
另外，通过共转染和筛选纯化获得了携带appa基因的重组家蚕杆状病毒。 |
| 4. | Sds - page analysis of pcr product of the recombinant virus showed with the amplified fregment was in conformity with the expected size , suggesting correct recombination and cloning of the ge gene
对重组病毒进行pcr鉴定，电泳结果显示扩增片段的大小与预期值相符， ge基因重组、克隆正确。 |
| 5. | The recombinant virus adhuctla4 - ig prepared in this study efliciently inltcted l - o2 " cells , and the infected cells expressed a - nd excreted soluble rccolllbina11t protein huctla4 - ig
该重组病毒在体外能有效感染正常肝细胞株l - o2 ，受感染细胞能表达、分泌可溶性的重组融合蛋白huctla4 - ig ； 2 |
| 6. | Furtherly expression phase analysis showed that orf2 appeared in different form during infection . to reveal the function of haorf2 , vha - orf2 - gfp recombinant virus which tagged with egfp - orf2 fusion protein was generated
构建了ha2与绿色荧光蛋白融合表达的重组病毒vha - orf2 - gfp ，对该重组病毒感染细胞后，融合篡硕士学位论文mast卜r ’ s ’ r拼ifs |
| 7. | The polyhedrin gene sequence in recombinant virus bmpak - hbmp can be more benefit to enhancing the expression of the hbsag ( pres2 + s ) from atg initial codon at the 5 " ends of pres2 gene
另一方面， elisa检测表明， presz抗原性提高率约是hbsag的14倍，提示bmpak hb帅中多角体基因部分碱基序列的存在更有利于从presz前的atg开始表达中蛋白全基因（ presz s ） 。 |
| 8. | Then bm cell line was co - transfected with parental bm - npv dna and the transfer plasmid pvl - nk , the recombinant virus was then obtained subsequently with routine procedure . nattokinase was expressed in silkworm larva by injection with the recombinant virus
将nk基因克隆至转移载体pvl - 1393中后用常规方法获得重组病毒，感染家蚕后纳豆激酶获得正确表达。 |
| 9. | To study the localization of the peptide , we also generated recombinant viruses containing the ctl - egfp fusion gene , following observation of infected larvae by such viruses will provide insight into the function of baculoviral conotoxinlike genes
为了研究毒素在虫体中的定位，构建了类蜗牛毒素和增强的绿荧光蛋白融合的重组病毒racbacctlgfp2和rhabacctlgfp ，为下一步研究奠定了基础。 |
| 10. | Pupal hemolymph infected with recombinant virus bmpak - hbmp and bmpak - hbm showed four protein bands of about 31 , ' 30 , 28 and 25 kd in the western blotting profiles , due to the two atg initial codons at the 5 " ends of both pres2 and s gene
Western杂交结果表明，感染bmpak hbmp和bmpak hbm的家蚕蛹血淋巴中均有4种不同分子量的蛋白质和鼠抗人的一抗发生免疫反应，大小约分别为31kd 、 30kd 、 zskd 、 25kd 。 |
