| 1. | Construction and immunogenicity of multi - epitopes recombinants
流感复合多表位疫苗的设计及小鼠免疫研究 |
| 2. | There are at least nine hiv subtypes in total , and several recombinants of these
目前至少有9种艾滋病病毒亚型，还有这几种亚型的重组类型。 |
| 3. | After identification with both pcr and enzyme digestion methods , accurate recombinants were sent to detect their sequences
将经过pcr和双酶切鉴定的重组克隆进行序列测定。 |
| 4. | But as to muts recombinants , the periods of fermentation is too long and the efficiency of using methanol is low
然而， mut ~ s菌株由于发酵周期长，甲醇率利用低，并不适用于实际的大规模生产。 |
| 5. | To find out the difference between mut + and muts recombinants we compared their expression of pokeweed antiviral protein in the same conditions
在相同的培养条件下比较了mut ~ +和mut ~ s重组菌株表达pap的异同。 |
| 6. | One of these recombinants by random chose was fermented . the target protein from the supernatant of bacteria lysate was purified
随机选取一株rhbfgf表达率约20并有相当可溶性蛋白表达的菌株进行大规模发酵、纯化。 |
| 7. | Then cdnas and puc18 vectors were linked by t4 dna ligase and transformed into e . coli strain dh5 - alpha to generate cdna library that size is 4 . 9 l06 recombinants
将cdna与载体连接，并导入dh5感受态细胞中，构建成cdna文库。 |
| 8. | The library size is 1 . 2x 106 recombinants . the average length of inserted fragments in the library was longer than 450bp , which was testified by both pcr and sequencing analysis
蓝白斑检验结果表明cdna文库滴度为1 . 2 10 ~ 6 ，重组效率为95 . 9 ， pcr检测及序列分析结果验证插入片段平均长度大于450bp 。 |
| 9. | They were cloned to pmd 18 - t respectively and selected two positive recombinants at random to analysis their sequences respectively , and compared their genes and amino acids with some other mammals
将它们分别克隆至pmd18 - t ，随机挑选几个阳性重组子进行测序。将测序结果与绵羊等多种哺乳动物该基因及相应的氨基酸进行同源性比较、分析。 |
| 10. | Using pet - 3c as vector , the cloned rhbfgf genes were expressed in bl21 ( de3 ) plyss . most rhbfgf were expressed in e . coli 10 % of total cellular protein . and 5 recombinants have much soluble rhbfgf
将这二十种重组子分别转化大肠杆菌bl21 ( de3 ) plyss ，进一步筛选得到16株表达量不等的菌株，其中有绝大部分表达率10 ，且有5株可溶性rhbfgf的表达较高。 |
