| 1. | Refolding and high - level expression study on human interferon - in e . coli
在大肠杆菌中高效表达和色谱复性研究 |
| 2. | Expression and refolding of a hla - a 0203 - bsp fusion protein and identification of its tetramers
的表达和复性及其四聚体的鉴定 |
| 3. | To study the renatural condition of r hpf4 , we adopted air oxygenation method to make r hpf4 refolding correctly
研究重组hpf4的复性条件，采用空气氧化法使重组hpf4正确重折叠。 |
| 4. | Various strategies , including the direct dilution method , have been utilized to enhance the performance of the refolding process ; however , refinement and improvement on these methods are ongoing
直接稀释法是复性程序中最简单的方法，但如何提高其复性效率仍在持续研究中。 |
| 5. | Finally , a combined scheme was proposed to optimize the refolding process , this work demonstrates the feasibility of applying fed - batch and / or step - addition operation schemes to the renaturation of enzyme activity
最后本研究中，结合馈料式与间歇式复性法之操作策略，可更有效率地提高其复性效率。 |
| 6. | Subsequent refolding step was optimized for a maximal recovery of biological active protein . the antiangiogenic activity of the hpk - 5 was estimated using a chorioallantoic membrane ( cam ) model of chicken embryos
4 .鸡胚绒毛尿囊膜实验结果显示， hpk一5能有效的抑制鸡胚绒毛尿囊膜新生毛细血管的生长。 |
| 7. | The aggregate of egg white lysozyme molecules formed during the refolding procedure of denatured - reduced egg white lysozymes was analyzed by protein electrophoreses and high - performance size - exclusion chromatography
本文利用蛋白电泳和高效凝胶排阻层析法分析了还原脉变性蛋白溶菌酶稀释复性过程中的集聚体。 |
| 8. | The aggregate of egg white lysozyme molecules formed during the refolding procedure of denatured - reduced egg white lysozymes was analyzed by protein electrophoreses and high - performance size - exclusion chromatography
摘要本文利用蛋白电泳和高效凝胶排阻层析法分析了还原脉变性蛋白溶菌酶稀释复性过程中的集聚体。 |
| 9. | Our research shows that the fed - batch and the step - addition schemes overcame the aggregation formation and favored the refolding step at higher feeding concentrations in comparison with the typical dilution method
由实验结果显示，馈料式与间歇式复性法相较于稀释法已有效地避免聚集体之生成及提高最终的操作浓度。 |
| 10. | Another chimera rgd - hw did not showed activity of platelet adhesion and aggregation that rgd sequence is thought to possess after complete refolding and purification , which explained that the resign and the progress of refolding of chimera rgd - hw was required improvement further
另外一个突变体rgd一卜铆经完全复性及分离纯化后没有显示出rgd序列应具有的抗血小板聚集活性，说明这个突变体分子设计和氧化复性过程还有待进一步优化。 |
