Studies on virus resistance of transgenic tobacco with tmv replicase gene 转烟草花叶病毒复制酶基因烟草的病毒抗性研究
2.
Complete sequence analysis of the two plasmids reveal that the small plasmid plp2000 with 2061 nucleotide encodes a putative 37 kda replicase . and in the downstream region of the replicase gene , it existed multi - tandem repeat of 17 bps Southernblot显示plp2000和plp9000两种质粒属于滚环复制类型质粒，和滚环复制相关的钻序列可能形成的二级结构分别为sso和ssou类型。
3.
There is typical - 10 region , - 35 region and rbs sequence on the upstream of orf1 and on the upstream of the - 35 region there are four tandem repeats of 22 bps sequence , and similar sequence was found on the upstream of the replicase genes of pmbbl , pvs40 , plu510 在orf1上游具有典型的- 10区、 - 35区和rbs碱基序列， - 35区上游附近四段22bp正向串联重复序列，相类似的序列也见于质粒pmbb1 、 pvs40 、 plq510复制蛋白酶基因上游。
4.
The detection of ribozyme gene with two cleavage sites that cleaves plrv replicase gene were present in the second part of this paper . a conserved sequence of 35s promoter of plrv was found in gene pools . two primers were designed based on the conserved sequence and bamh i and ribozyme gene . the genomic dna of potato was amplified by the primers through polymerase chain reaction ( pcr ) 从许多资料中报道的plrv的35s启动子的序列中找出一段保守序列，然后根据与核酶紧密相连的bamh和这段保守序列设计两段引物，用这两段引物通过pcr扩增转基因马铃薯的基因组dna ，并进行检测。
