| 1. | Successive sampling survey ; retain sample ; matching ratio ; rhc sampling design 连续抽样保留样本匹配比rhc设计 |
| 2. | The board will decide whether a rhc should be appointed to hear the representations 城规会会决定应否委出一个聆讯申述小组委员会,聆讯申述个案。 |
| 3. | Each rhc consists of a chairman currently the director of planning , a deputy chairman a non - official member , two official members and five non - official members 每个聆讯申述小组委员会的成员包括1位主席目前为规划署署长1位副主席非官方成员两名官方成员及5名非官方成员。 |
| 4. | We also conducted the co - transfection with the rnai plasmids rhe and rhc and the selective marker gfp expressing plasmids simultaneously . there are three kinds of combination probability that could be observed by gfp marker 在实验中同时共转染人集缩素smc亚基hcap一e和hcap一c特异的rnai质粒rhe和rhc和pcdna3 . 1 + kg到hela细胞。 |
| 5. | This report explored the function of the human smc subunits - hcap - e and hcap - c using rnai and transfection techniques . we constructed two rnai plasmids - rhe and rhc that specifically corresponded to hcap - e and hcap - c of human condensin , respectively 为此我们构建了对人集缩素中两个smc亚基hcap - e和hcap - c特异的rnai质粒rhe和rhc 。 |
| 6. | They are combination of co - transfection with rhe and gfp expressing plasmids , co - transfection with rhc and gfp expressing plasmids and co - transfection with plasmids rhe and rhc and gfp expressing plasmids at the same time . whichever the combination may be , we always observed the phenomena of chromatin bridge in co - transfected cells . these results indicate that the phenomena of chromatin bridge should be the characteristic feature of deficiency in the human smc subunits 这时,存在三种可能的情况,即共转染rhe和pedna3 . 1 * kc 、共转染rhc和pedna3 . 1 + kg 、共转染rhe和rhc和pc洲a3 . 1 + kg ,但都观察到明显的染色质桥现象,进一步证明这种表型是人集缩素中smc亚基的缺陷的特征表型。 |
| 7. | The individuals of rhd - positive phenotype with intact exons carried generally insert fragments and boxl box2 and box3 and this proved that inserts or rh box could n ' t affect the express of rh d gene . in 2 of the 5 wei nationality pedigrees whose proband were rh d - negative , rhc / e phenotype of all the rh negative individuals was ccee . rhd exon 4nsert and rh box did not be found in all individuals 在7个先证者为rhd阴性的汉族家系中,大部分成员均出现插入片段和rhbox ,且在遗传上符合孟德尔遗传定律, d外显子完整且表型为rhd阳性的家系个体成员广泛带有插入片段和box1 、 box2或box3 ,插入片段或rhbox并未影响d基因的表达。 |
| 8. | The effectiveness of the nuclear localization signal selected was clearly tested in initial experiments . based on this , the co - transfection systems used in the study was established . we first observed the multinucleate cells and chromatin bridges in cultured hela cells when the cells are marked with gfp and hochest33342 , after co - transfection with the gfp expression plasmids and rnai plasmids rhe or rhc 当分别共转染附加kozac序列和核定位信号的gfp与人集缩素smc亚基hcap一e特异的rnai质粒rhe和人集缩素smc亚基hca尸一c的f { nai质粒rhc时,都观察到gfp和hochest33342标记的转染后hela细胞表现多核和染色质桥现象。 |