Compared with ribonuclease a , the ribonuclease activity of ang is very low , but is very important to promote new endothelia proliferation Ang的rnase活性很低，但它裂解rna的活性对于它的血管生成活性非常重要。
2.
Angiogenin ( ang ) is a small monomer which universally exists in tissues and cells . angiogenin belonging to ribonuclease superfamily has 35 % homologous sequences with ribonuclease a ( rnase a ) 血管生长因子( angiogenin ， ang )是一个小的单聚蛋白，与rnasea是同系物，在组织和细胞中分布广泛。
3.
This modification includes : ( 1 ) selecting two important molecules as candidates , ( 2 ) choosing a promiscuous t - cell epitope , and two b - cell epitopes or conserved amino acid sequences from the two important molecules , ( 3 ) connecting them adequately through analysis by the molecule designing software . therefore , the synthetic new antigen may interfere with the process of fertilization by multiple ways and its contraceptive effects may be enhancing . based on the molecule designing methods , the b - lymphocyte cell epitope of sperm / testis specific protein sp17 and cyritestin which interfere with fertilization in mouse , as well as the promiscuous th cell epitope of the ribonuclease ( rnase ) in bovine were selected 本研究以蛋白质分子设计的理论和方法研究避孕疫苗，将sp17和cyritestin关键表位和牛核糖核酸酶非选择性th细胞表位合理组合，获得新抗原- 35肽序列；并在合成、纯化后分别与弗氏佐剂、免疫刺激复合物( iscoms )混合后免疫不同遗传背景的雌性小鼠，观察血清和生殖道内的特异性抗体滴度的动态变化、生育力的改变以及免疫后小鼠重要脏器的组织病理学改变：以及在ivf下，新抗原的特异性抗血清对精卵相互作用的影响及抗原在精子表面的特异性定位。
4.
We designed a suitable probe in the experiment and used ribonuclease protection assay technology to detect the expression of pdipl gene in mouse liver at different time points after partial hepatectomy . we found that the expression of pdipl gene was increased . it reached the highest level after 12 hours and then decreased 我们设计合适的探针，采用核酸酶保护反应等技术测定了pdip1 -基因在肝脏部分切除后不同时间在小鼠肝脏中的表达，发现pdip1 -基因在肝脏部分切除后表达增强， 12小时表达最强，以后表达减弱。
5.
Ribonuclease inhibitor ( ri ) is an acid cytoplasmic protein with the molecular weight of 50 kd . this ubiquitously distributed glycoprotein tightly binds and inhibits ang with a stoichiometry of 1 : 1 . the remarkable affinity of ri and ang supplies an approach to invalid the function of ang which is to stimulate neovascularization 核糖核酸酶抑制因子( ribonucleaseinhibitor , ri )能够以1 ： 1的比例结合并抑制ang ，这无疑为我们提供了一条重要的纠正ang引起的新生血管生长失控的途径，从而治疗相关疾病。
