| 1. | Ribozyme structural elements : hairpin ribozyme
核酶结构元件 |
| 2. | Ribozyme structural elements : group i introns
核酶结构元件 |
| 3. | A specific cis - hairpin ribozyme facilitates the infection in tobacco protoplasts
一个特殊的顺式发夹核酶对感染烟草原生质体的影响 |
| 4. | The egs can be covalently linked to ml rna , the catalytic rna subunit of rnase p , forming a new kind of ribozyme , m1gs
将egs共价连接到m1rna的3末端成为附属于m1rna的一段引导序列，称为gs 。 |
| 5. | Construction of an expressing vector for antisense rna - ribozyme chimeric dna sequence against tomato acc synthase gene and transformation of tomato
核酶嵌合基因植物表达载体的构建及对番茄的转化 |
| 6. | As indicated above , several m1gs that cleaved hcmv ul54 mrna segments in vitro were successfully designed and constructed . our studies demonstrates the utility of this ribozyme m1gs for antiviral application
我们的研究成功地利用引导序列，将核酶rnasep催化亚单位m1rna构建为序列识别的核酶m1gs ，证实了核酶在抗病毒方面的应用价值。 |
| 7. | Sequencing report of pcr product shows the ribozyme gene with two cleavage sites has already integrated into the genome of potato . and it also proved 35s promoter of prok2 was same as that of hajdukiewicz , p . and rna detection is going on
检测结果证明：所扩增的dna包含核酶基因反转录后的dna ，证明核酶基因已被转入马铃薯j - 1中；同时证明了转基因的prok2的35s启动子与hajdukiewicz ， p等所公布的启动子相同。 |
| 8. | Constructing human colorectal cancer cell line with stably down - regulated grp94 ( 1 ) the plasmid prc / rsv - ribol that contains specific grp94 - targeting ribozyme and the control plasmid prc / rsv were miniprepared , respectively , cleaved by endoenzyme pvuii
稳定下调grp94的人大肠癌细胞克隆株的构建( 1 )分别对含有特异性打靶grp94核酶的质粒prc rsv - ribo1和对照组质粒prc rsv进行小量提取、 pvu酶切鉴定。 |
| 9. | The fragments were subcloned into a low copy transcription vector ( px8dt ) between the t7 rna polymerase promoter and autocatalytic hepatitis delta virus ribozyme . the result showed that genome of ndv f48e9 strain comprises 15192 nt , which was equal to zjl strain and 6 nucleosides longer than that of la sota , v4 , bl and clone30
实验结果表明： f48e9全基因组具有15192个碱基，比lasota 、 v4 、 b1和clone30的全基因组序列长6个碱基，和鹅源zj1株的长度相等。 |
| 10. | Based on this consideration , we chose human colorectal cancer cell line ccl229 as the experimental object , down - regulating its grp94 expression by specific ribozyme targeting . we observed its effects on the biological characteristics of ccl229 and upr pathway , and gained further understand of the grp94 , upr and tumor relationship
基于此，我们选择了人大肠癌细胞株ccl229作为研究对象，通过特异性核酶打靶的方式，下调细胞中grp94的表达水平，观察其对人大肠癌细胞某些生物学特性及upr通路的影响，加深对grp94 、 upr和肿瘤这三者间关系的了解。 |
