English translation for "saci"

非洲工商业公司
萨西

Related Translations:

saci aziza:  萨西阿齐扎

saci lamouri:  萨西拉穆里

Example Sentences:

	1.	On the base of construction of pbi121vp7 , we constructed the fusion gene expression vector pbi121ctbvp7 by the same sigle cloning site ( ndei and xbai ) of vector puc19ctb and pbi121vp7 设计具有ndei和saci单限制性酶切位点的vp7基因引物，通过pcr扩增出vp7基因并经测序验证。
	2.	And wibdv specific rt - pcr also uesed to detected these samples in the years from 1993 to 2000 , there are 8 strains belong to wibdv and 13 belong cibdv . two strains ca n ' t typing for they can be cleaved with both of enzymes sspl and saci . 结果发现1993年至2003年的病料或分离的毒株中，有8株确定为vvibdv ， 13株踊定为cibdv ，另外，有两株不能确定。
	3.	Dna band is about 600 bp when p1asmid was digested by sacii and saci enzyme , and is equal to that of hbsag gene ; but it is about 700bp when plasmid was digested by xhoi and saci enzyme , and is equa1 to the weight of s , . / hbsag fusion gene 经sacll和saclk切电泳可见约600hp的dna带，与hbsag基因大小相当；用xhol和sacl切电泳可见约700hp的dna带，同s …与hbsag基因融合片段的大小相等说明s ； 。
	4.	But the vvidbv strain gx8 / 99 that had been identified by gui only have saci side , and no sspl , the reason need farther research . according to the specific different sequence between cibdv and vvibdv in vvp2 gene . we also designed two sets of primers ( cibda + cibds and vvibda and vvibds ) 另外，针对不同强弱毒株的1 v的pz高变区序列的差异：设计合成了vvibdv的型特异性引物（ ibda vvibds人建立型特异的rt pcr ，只需一次反应即可区分cibdv和vvibdv 。
	5.	Construction and expression of yeast engineering yaccine : s14 / gnsag " as transformed to yeast host straln x33 by means of electroporation after ppiczaa s , . aresag " as l ined by saci enzyme . the single fungus , as choose and dibble inocu1ating in we and am plate , the positive fungus was gro ' ing in rm but not in w , and was 6 inoculated in ypd which included zeocine 500ug / ml and 1000ug / ml . 5 transformers were ampl if ied by pcr , three is same with positive control 选取单个菌落分别点种到删平板和md平板，找出在回d上生长正常， w上生长缓慢或不生长的菌落，即阳性菌落，再以阳性菌落分别涂布zeocine含量500ug加， 1000ug砌l的ypd平板，以高浓度的抗生素筛选高拷贝的酵母工程菌，在含500ug ml高浓度抗生素平板上获得了15个转化子，取其中5个进行pcr扩增，有3个扩增产物与阳性对照相同，说明此酵母细胞中已含有s hbsag融合片段，其中之一命名为p
	6.	Correct clones were selected and plasmid dna was isolated and digested with saci and puvii . a dna fragment of about 2 . 1kb was purified and labeled by dig - 11dutp as probe . at least 40 positive clones were obtained from human genomic library by in situ colony hybridyzation with this probe . among them one clone contains human serum album dna by sequence 以pcr扩增的人血清白蛋白( hsa )基因片段为探针，从人的基因组文库中杂交筛选的阳性克隆中，经测序分析，有一个克隆含有全长hsadna ，用从其它的阳性克隆中选取两种dna片段，即dna修复基因hfen1和一段非编码大片段cit987sk - 384d8 ，与人hsadna一起，进行显微共注射，成功制备了转多基因小鼠。
	7.	By the same method , the expression vector pbi121 - cp was constructed from pgem - cp and pbi121 with xbal and saci digestion . after that , the pbi121 - cp was transferred into agrobacterium lba4404 strain by freeze - thaw method . the pcr amplification indicated the lba4404 strain containning cpti gene . the lba4404 strain was used in genie transformation of mustard 经酶切和pcr扩增验证后，以xbai和saci双酶切pgem ? cp和pbi121 ，将切下的cpti片段和pbi121载体片段连接构建成表达载体pbi121 - cp ，用冻融法导入农杆菌lba4404 ，提取质粒，经pcr扩增检测，用于芥菜的遗传转化。
	8.	Over 13 kb saci restriction fragment was cloned into pgem - 7zf ( + ) , mapped for restriction endonuclease sites and an about 5 . 0kb fragment was further subcloned and sequenced . through coding region specific primer , we amplied it ' s corresponding cdna , named st901 . st901 is 2889bp long , contains 1447bp putative promoter region within 5 " upstream and three exons ( 475bp , 140bp , 39bp ) and two introns ( 472bp , 2s3bp ) in the coding region , encodes a hydrophilic protein of 217 amino acid residues with a molecular mass of 24kda 以同源探针筛选马铃薯基因组文库，得到四倍体马铃薯基因组dna ? st901 ，基因全长2889bp ，含3个外显子(长度分别为475bp ， 140bp ， 39bp )和2个内含子(长度分别为472bp ， 253bp ) ； 5 ’端含有1447bp的启动子区段，该区段具备一般启动子的基本元件tatabox和caatbox ； 3 ’非编码区长63bp ，具hind酶切位点，没有发现保守的加尾信号。
	9.	Two useful restriction endonucleases ( sspl and saci ) were choosed to type the different pathgenic ibdv strains . the result is saci only cleaved cibdv ( 4vaccine strains : bj836 , b87 , d78 , bdc and 6 standard cibdv strains : hel , he2 , he3 , he4 , sd3 / 98 , zj1 / 98 ) and vibdv ( american variant - e ) rt - pcr products , whereas products obtained with vvibdv strains ( yl1 , yl2 , yl5 , ylz ) were only cleaved with sspl 选用具有分型意庐卢人耸2003届硕十学位论文2义的两种限制性内切酶（ saci和sspi ）建立的rea ，对5株属于cibdv的疫苗株和6株标准强毒株；属于vvibdv的毒株gx 、 yli 、 ylz和ylz等分离毒：属于叶v的美国变异e株进行酶切分析，结果与前人的研究相符。

Similar Words：

"sachuda" English translation, "sachumpa" English translation, "sachunsky" English translation, "sachurov" English translation, "sachverhalt" English translation, "saci aziza" English translation, "saci lamouri" English translation, "sacic" English translation, "sacid green g" English translation, "sacido" English translation