| 1. | Advances in studies on saussurea involucrata 雪莲的研究进展 |
| 2. | Saussurea involucrate ' s ecological properties and reproductive techniques 雪莲花的生态学特性与繁殖技术 |
| 3. | Cloning and sequence analysis of chi gene fragment from saussurea medusa maxim 与以公布的chi序列orf完全一致。 |
| 4. | Comparison of the contents of total flavone between artificial cultivated and wild saussurea involucrata 野生与种植雪莲花的总黄酮含量比较 |
| 5. | Determination of the conctents of saussurea lappa essential oil in chinese traditional medicine po chai pills by hplc 测定中成药保济丸中木香挥发油的含量 |
| 6. | Influence of total flavone in culture of saussurea medusa maxim and the wild one on inflammatory factors in blood serum of inflammation model rat 水母雪莲总黄酮和水母雪莲细胞培养物总黄酮对致炎大鼠血清中炎性因子的影响 |
| 7. | In this dissertation , the author cloned the chi gene from saussurea medusa maxim by rt - pcr , and constructed the chi gene sense and antisense plant expressional vector pbinchi + and pblchi . , 论文研究内容与结果如下: 1水母雪莲chi基因的克隆及其序列分析以水母雪莲( saussureamedusamaxim |
| 8. | The chi gene fragment was amplified by means of rt - pcr , using flower mrna of saussurea medusa maxim , as template , and a pair of specific oligonucleotides ( according to the chi gene sequence , af509335 ) as primer , and cloned into pucm - t vector with ta connection . the vector was named pucm - chi Chi基因的正向和反向植物表达载体的构建用ecor和sal双酶切p ~ ( ucm - chi )和中间载体ptriplex2 ,回收、连接目的片段与载体,构建中间载体p ~ ( tripchi ) ,选择正向重组质粒。 |
| 9. | Saussurea medusa maxism was used by several minorities in china , because it have some special pharmaceutical effects such as detoxification , detumescence , abirritation , antitumor , anti - radiation and anti - ageing etc . but saussurea medusa maxism was on the edge of annihilation for being exploited excessively at a long time . still the natural resource of it is hard to meet the increasing need of market . so ths botanical scientist paid more attention to the relationship between protecting this endangered species and producing it ' s pharmaceutical ingredient 具有清热解毒、抗炎镇痛、祛风除湿,通经活络,壮阳补血、消肿、利痰、敛伤的功效。用于炭疽病、中风、风湿关节炎、崩漏带下、痛经、胎衣不下、肾虚腰痛、遗精阳痿等病症。近年来又发掘出如延缓衰老、抗肿瘤、抗辐射等更有价值的效用。 |
| 10. | In order to establish the genetic transformation system of saussurea medusa maxism by agrobacterium rhizogenes , some work were done . the main results were following : 1 establishment of regeneration systems two systems of regeneration from saussurea medusa maxim were established without cold treatment . the somatic embryos were induced from callus cultured in mise for 35 days . the shoots were induced from cotyledon after cultured in misc 20 days , and from leave which were cultured in misl . the experiment showed that the carbon and glycine in the medium could help to increase the regeneration rate to 95 % 为筛选水母雪莲代谢突变体和转基因研究奠定了一定的基础,陈亚琼: m质粒介导水母雪莲的遗传转化及毛状根中决删合成的调节p义摘要也为研究类黄酮代谢途径的关键酶基因的转化、高效表达及作用机理提供了理想的实验体系。主要的实验结果如下: 1水母雪莲高频再生体系的建立通过体细胞胚胎发生途径和器官发生途径,水母雪莲( squssureamedusamaxim )可以在常温下获得再生植株。 |