Leathers and hides . chemical testing of leather . calculation of degree of tannage 皮革.皮革的化学试验.鞣革程度的计算
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Leathers and hides . chemical testing of leather . examination for penetration of tannage 皮革.皮革的化学试验.鞣革的透入度检验
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Standard practice for calculation of non - mineral combined tanning agents and degree of tannage 非矿物组合鞣皮剂和鞣皮度计算的标准实施规程
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Leather - chemical tests - calculation of degree of tannage , leather substance and bond organic substance 皮革.化学试验.鞣透度革质及结合鞣质的计算
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Dna extraction from different specimens is the foundation of conservation genetics . it is difficult to extract total dna from tannage of chinese alligator with usual method 利用不同的实验材料提取适合研究的dna是保护遗传学研究的基础,而对于鞣制皮革,用常规的dna提取方法很难提到适合于研究的dna样品。
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The results showed that dna of tannage could be extracted by this method and used as samples in conservation genetics . it is a good idea for molecular identification of leather things and amplification or sequencing of genes 结果证明,运用这种改进的提取方法从鞣制皮革中提取的总dna适合保护遗传学研究的需要,可用于部分序列的扩增和测序研究及扬子鳄皮质用品等的分子鉴定。
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In this paper , total dna from tannage , tail skin , scales and the skin treated with salt were successfully extracted with an improved method for dna extraction . to verify the results , four pairs of primers , which were universal primers for 12s rrna gene , diagnostic primers of chinese alligator meat , microsatellite primers and rapd primer , were used to do pcr amplifications . some amplified fragments were sequenced , either 本文研究出一种改进的dna提取方法,成功地从扬子鳄鞣制皮革中提取了总dna ,同时对不同的组织标本如鳞片、盐腌生皮及尾尖皮等进行了dna提取:并利用12srrna基因扩增的通用引物、扬子鳄鉴定性引物、微卫星引物及rapd引物进行pcr扩增,且对部分pcr扩增结果进行测序,以检验提取效果。