Effects of tcdd on the embryo lost at early pregnancy and survive rate at born 四氯苯二?哄对小鼠胚胎毒作用的研究
2.
The effects of tcdd on pregnant cynomolgus macaque macacafascicularis and its pregnancy outcome 对猕猴妊娠期及其妊娠结局的毒性影响
3.
There were many growth factors taking part in the control and regulation of metabolism within the bone development . 2 , 3 , 7 , 8 - tetrachlorodibenzo - p - dioxin ( tcdd ) is a widely spread environmental contaminant with extremely toxic effect 在骨发育过程中，激素调节、生长因子调节与神经系统共同形成了神经-内分泌-免疫-局部生长因子调节网络，参与骨发育的整个调节过程。
4.
The laboratory animal studies on tcdd and ra exposure indicated high incidences of congenital developmental malformations . in our previously study , we analyzed gene expression profile in igf gene family of tcdd - and ra - reduced fetal rats as well as cells and found antiestrogenic effect of tcdd and ra on igf genes 我们应用ra对不同发育时期的胎鼠进行诱导，构建骨骼发育畸形大鼠动物模型，研究遗传因素决定的先天畸形个体间对环境因素作用的易感性，阐明igfs家族成员在骨骼发育畸形大鼠中的作用。
5.
The insulin - like growth factor - 2 ( igf - 2 ) and igf conjugated protein - 6 ( igfbp - 6 ) mrna level in rat calvaria bone tissue and mc - 3t3 - el cells were detected by northern blotting analyses and reverse transcription polymerase chain reaction . the estrogen responsive element ( ere ) in igfbp - 6 gene promoter was identified and involved in tcdd - reduced regulation of the gene expression by electromobility shift assays ( emsa ) 正常胎鼠头盖骨组织igf一2mrna呈高水平表达状态，而igfbp一6mrna的水平较低; ccf胎鼠头盖骨骨组织内igf一2mrna的表达较正常胎鼠降低， igfbp一6mrna的表达则明显升高; atra和e :联合应用时， atra可以抑制雌激素对细胞内igf一2和igfbp一6的这种调节作用。
6.
The protein nucleic acid complexes were identified by autoradiography for 48 h . the psg5heo and igfbp - 6 promoter pcat vectors were transfected into cos - 7 cells with iipofectamin2000 ; these cos - 7 cells where treated with 1 jxm e2 , with lonm tcdd and with both lonm tcdd and 1 jxm e2 , individually ; the cat protein was examined by cat - els a . results : the congenital skeletal defects in fetal rats were induced with 10 - 15ug / kg tcdd or with 100 - 140 mg / kg ra with dosage - dependence effect . the igfbp - 6 mrna in rat calvaria tissue and mc - 3t3 - el cells were increased with tcdd and ( or ) ra , whereas the cell proliferation and igf - 2 mrna in mc - 3t3 - el cells was decreased in the status of e2 结果: tcdd在大鼠胚胎发育时期具有骨骼致畸作用，骨骼发育畸形包括:内翻足、脊柱裂、颅面骨发育缺陷、无尾畸形等;随着tcdd浓度的升高( 10一15林扩kg ) ，致畸率明显上升( 14 . 7 %一24 . 6 % ) ; tcdd和ra联合用药可以增强对大鼠骨骼发育的致畸效应; e :对成骨细胞内igf一2基因有正性调节作用，对igfbp一6基因有负性调节作用， tcdd可以抑制e :对细胞内xgf一2和igfbp一6的调节作用。
7.
The ratio of ( s + g2 ) / ( g2 + m + s ) and pi value of mc - 3t3 - el cells increased in the condition of 1um / l e2 . the effects of e2 on igfbp - 6 and igf - 2 gene expressions were suppressed by tcdd and ra . the binding of ere at higfbp - 6 5 " - utr ( dna sequence : aactctgacc , + 94 to + 103 ) was enhanced by tcdd 第三部分igfbp一6基因5 ’一utr中ere的鉴定及功能分析目的:骨骼组织起源于胚胎期中胚层，维持正常的胚胎期骨骼发育需要多种生长因子、细胞因子和激素的参与，其中雌激素可通过多种途径作用子中胚层内间
8.
Tcdd influence the activity of diverse hormones , hormone receptors , mitogens , and other biological response modifiers and these interactions undoubtedly play an important role in the toxicity of these response . retinoic acid ( ra ) is ramification of vitamin a , having extensive biological effect on controlling conformation genesis , proliferation , differentiation , growth , metabolism of kinds of tissues and cells and stabilization of internal environment 第一部分igfs家族成分在维甲酸诱导马蹄内翻足畸形中的调控机制目的：维甲酸( retinoicacid ， ra )类物质是维生素a的衍生物，在调控多种组织和细胞的形态发生、增殖分化、生长发育、代谢及维持内环境的稳定等方面具有广泛的生物学活性。
