| 1. | We find the new cloned fusion protein has inhibitory effect on the activity of tpk
Sc4b亲和层析纯化后，得到纯度较高的重组蛋白hbrp 。 |
| 2. | Covering 13 , 300 sq - m area , tpk locates on the amoy export and import processing zone in haicang area
公司占地面积达到了13 ， 300平方米，位于厦门市海沧进出口加工区。 |
| 3. | The enterprise of tpk kapfer gmbh offers you microswitches , reporting devices and plug sockets , power points as well as the further signal devices and mine counter ( unclear )
Tpk kapfer gmbh是一家高效的工业型企业，企业生产微型开关,信号议,信号装置,插座范围内高水平的产品。 |
| 4. | As we know tpk is the important key enzyme in cell growth and metabolism . so what we have done is of great significance to study further the biological functions of the new cloned protein related to bsp proteins
我们从大鼠子宫中通过deae 52离子交换柱等纯化得到tpk样品，收集有活性部分，一切操作在4t中进行，然后观察通过基因工程得到的hbrp对tpk活性的影响。 |
| 5. | In both cell lines ex - pressing sh2a gene , the cytoplasm pkc activity decreased 40 % , but no apparent alteration was found in mapk , tpk and membrane pkc activity . the proliferation index of transfected cells exhibited an decline in cytometry detection
L sh的肝癌bel7402细胞及cos7细胞胞浆蛋白中的pkc活性均下降达40 ，胞膜pkc 、胞浆mapk ， ’ fpk活性未见明显改变，转染细胞经流式细胞仪检测增殖指数有所下降。 |
| 6. | This guide is one of the eyes on nature series , providing information about the fauna of tai po kau nature reserve . it also provides a useful reference for mammal sightings made throughout the territory , since the animals described are not confined only to tpk
本指南是放眼大自然系列丛书的其中一册，旨在提供大浦?自然护理区内的哺乳类动物资料，但由于其中述及的物种并不限于在大浦?出没，因此也可用作观察全港哺乳类动物的参考书。 |
| 7. | Multiple kinase assay was performed to examine pkc ^ mapk . tpk activity in the transfected cells . meantime , pegfp - sh2a vector was also constructed and the cells transfected with it were examined by fluorescent microscopy . the expression of sh2a gene was examined under different concentration and time of bfgf as a stimulating factor
1 sh ，利用脂质体转染肝癌bel7402细胞人os7细胞，检测pkc 、 mapk 、 tpk活性的改变；流式细胞仪检测细胞增殖；另构建pegfp sh ，转染细胞，荧光显微镜观察荧光定位； bfgf作为刺激因素处理细胞，根据不同浓度、时间检测sh基因表达情况。 |
| 8. | These suggest the bsp proteins and its homologous proteins are ubiquitous in mammals and must have some physical significances . in our research , to find and study the important role of bsp proteinsand their related proteins in the development of zygotes and to find the new gene related to bsp , we cloned a cdna gene sequence related to bsp proteins and make it express in eukaratic cells . the fusion protein acquired are purified are used to deduce its biological functions
而在我们的实验中为了发现和研究bsp及其相关蛋白在受精卵的发育中的重要作用，寻找bsp相关的新基因，克隆了一个与bsp蛋白相关基因的山一na序列并将该基因编码的蛋白质在原核细胞中进行了表达和纯化，将纯化后的融合蛋白作了一些生物学功能方面的初步工作，发现新蛋白对tpk有一定的抑制作用，而tpk是细胞生长和代谢中十分重要的关键酶，这对于进一步研究新蛋白的生物学功能具有十分重要的意义。 |
| 9. | Materials and methods in our study , first the e . coli bl21 transformed already are cultivated in smaller scale and then the plasmids dna were extracted by the methods of alkaline lysis . the plasmids dna extraction were processed 37 overnight by the restrictive endo - incisase bam h i and xho i . the incision products were used to check the integrality and variability of the recombinant plasmids dna by 1 % agarose gel elec - trophoresis
同预想结果基本吻合，大规模培养后纯化得到的融合蛋白通过sds page显示在52kda处有一条带，而酶切后产物电泳结果显示在26kda处分另有两条蛋白带，其中一条为gst ，另一条为hbrp ，基本符合实验结果； hbrp对tpk活性的抑制呈剂量依赖性。 |
