In order to enhance the trehalose production , y7 was used for mutation with ems . through primary screening and compound screening , strain l61 with high - yielding trehalose and deep neutral trehalase activity was screened 实验对出发菌株y7进行了ems诱变处理,通过初筛、复筛,从中选育出一株能高产海藻糖同时具有较低海藻糖分解酶活性的菌株l61 。
2.
Comparing with the y7 , the specific growth rate of mutant increased by 22 . 7 % , the activities of neutral and acid trehalase droped by 19 . 2 % and 6 . 7 % , respectively . under the non - optimized condition , the production of trehalose increased by 57 . 2 % compared with that of parental strain y7 与原菌株y7相比较,对数期比生长速率提高22 . 7 ,酸性海藻糖分解酶活性下降19 . 2 ,中性海藻糖分解酶活性下降26 . 7 ,胞内海藻糖含量提高了57 . 2 。