Preparation and application of monclonal antibodies against trypsinogen activation peptide 抗胰蛋白酶原激活肽单克隆抗体的制备及应用
3.
For protein identification , proteins spots of interest on the gels stained with colloidal coomassie brilliant blue g - 250 were excised , digested in - gel with trypsin , and analyzed by peptide mass fingerprinting ( pmf ) with matrix assisted laser desorption / ionization - mass spectrometry ( maldi - ms ) . erp60 , trypsinogen , proelastase , lipaseandso on were identified . this will enable us to present an overview of the proteins expressed in rat pancreas tissues and lays the basis for subsequent comparative proteome analysis studies with pancreas development 从中随机选择一些差异蛋白质点，进行基质辅助激光解吸-电离飞行时间质谱( maldi - tof - ms )测定其胶内酶解后的肽质指纹图谱，用mascot软件查询swiss - port数据库，初步鉴定为一些与生长发育、物质代谢、细胞因子、信号转导等有关的蛋白质，如erp60 、 trypsinogen 、 proelastase 、 lipase等。
