| 1. | Our research was mainly focused on the transcription pattern of xist in preimplantation mouse embryos .
但是由于胚胎发育早期xist转录本数量的稀少而难以达到很好的效果。 |
| 2. | When the annealing tempreture of 55 has been determined , the nestled rt - pcr method was used to analyze the transcription pattern of xist
最终将其反应条件确定为退火温度55 ， sry和xist可以置于同一体系中扩增。 |
| 3. | Xist is an important gene wich is located in the xic ( x chromosome inactivation center ) region and is closely related to the x chromosome inactivation
X染色体失活与位于“ x染色体失活中心” ( xic )上xist基因息息相关，主要表现在xist的转录产物通过包围一条x染色体使其沉默。 |
| 4. | The gene silences the xi chromosome by spreading along the xi and inhibits most of its gene transcription . . due to the important role it played in the inactivation process , xist is gradually becoming a hot spot .
由于其在x染色体失活中的重要作用，对xist的研究一直是近几年的研究热点。其中很重要的一点就是试图建立xist染色体在发育过程中的时空转录模式。 |
| 5. | We employed the rt - pcr method to investigate whether xist is transcribed in some of the developing stages of mouse . first , different mouse tissues which have been proved to transcribe xist and sry simultaneously were used to establish the appropriate conditions for xist and sry rt - pcr
首先提取了6周龄昆明白小鼠几个组织的rna ，用普通反转录法扩增xist和sry以确定rt - pcr的条件， sry的主要作用是用来做性别鉴定。 |
| 6. | By establishing the transcription pattern of the preimplantion embryos , this experiment not only helped to explain some of the x chromosome inactivation phenomena , but also furnished the researcher who were dedicated to the research of reprogramming of somatic cloning and preimplantation development with useful materials and references
本论文不仅建立了xist基因在小鼠胚胎早期发育过程中的转录模式，为解释x染色体的失活提供了理论依据，而且，也为体细胞克隆胚胎的重新编程和早期胚胎的发育提供了有益参考。 |
| 7. | Using the hprt gene as a positive control , our result suggested that both the testis tissue and the male embryos from which sry transcription can be detected failed to yield any positive results of xist . female embryos at the pronucleus stage and 2 - cell failed to produce any positive result of sry and xist too . while since the 4 - cells period , xist is constantly transcribed until blastocyst stages
然后利用实验一确定的pcr条件，以hprt为阳性对照，用巢式rt - pcr对小鼠早期胚胎进行xist基因的转录分析，结果发现，转录sry基因的睾丸组织以及雄性胚胎，从受精卵发育到囊胚的过程中，基本上不转录xist基因；不转录sry基因的雌性卵母细胞和雌性胚胎，从出现原核开始，到发育至2 -细胞期的过程中， xist基因一直不转录，但是，从4 -细胞期开始，一直到孵化前囊胚阶段，雌性胚胎都转录xist基因。 |
