English translation for "ypd"

粉胨葡萄糖琼脂培养基

Related Translations:

ypd pile driver:  打桩船

Example Sentences:

	1.	Multicopy clones were screened from vary levels of g418 - ypd plate and cultured and induced with methanol to secrete interesting peptide , which was identified by sds - page and western blotting 培养多拷贝插入的酵母细胞，用甲醇诱导目的蛋白的分泌， sds - page和westernblotting鉴定表达蛋白。
	2.	The transfermants with highest resistance to g418 ( 4 g / l in ypd ) were screened to study their expression level in 150 ml shaking flask . having been induced with methanol for 36 hours , the target enzyme could be examined in the supernatant by measuring amidolytic activity 首次将大连蛇岛赅蛇毒类凝血酶成熟基回克隆到表达载体ppicgk中，经电激转化至毕氏酵母菌株gs15中，再经甲醇诱导，在150ml摇瓶畔1获得细胞外分泌表达产物。
	3.	Construction and expression of yeast engineering yaccine : s14 / gnsag " as transformed to yeast host straln x33 by means of electroporation after ppiczaa s , . aresag " as l ined by saci enzyme . the single fungus , as choose and dibble inocu1ating in we and am plate , the positive fungus was gro ' ing in rm but not in w , and was 6 inoculated in ypd which included zeocine 500ug / ml and 1000ug / ml . 5 transformers were ampl if ied by pcr , three is same with positive control 选取单个菌落分别点种到删平板和md平板，找出在回d上生长正常， w上生长缓慢或不生长的菌落，即阳性菌落，再以阳性菌落分别涂布zeocine含量500ug加， 1000ug砌l的ypd平板，以高浓度的抗生素筛选高拷贝的酵母工程菌，在含500ug ml高浓度抗生素平板上获得了15个转化子，取其中5个进行pcr扩增，有3个扩增产物与阳性对照相同，说明此酵母细胞中已含有s hbsag融合片段，其中之一命名为p
	4.	The recombinant plasimid ppic9k - pzp3 a - hcg p - ctp109 - 145 was transformed into electroporated pichia pastoris and screened the positive strains on md plates without histidine . multi - copy recombinants were screened on the g418 - ypd plates and then were incubated with the supernatant containing methanol . the recombinant pzp3 a - hcg p - ctp 109 - 145 protein were secreted in the supern atant and were verified 转化嗜甲醇酵母获得耐受4mg mlg418的菌株，经sds - page电泳和westernblotting检测表明其上清培养液中的蛋白可与猴抗pzp3抗体和兔抗hcg抗体发生特异性的反应。
	5.	The objective of this research is to transform the cloned phytase gene into pichia pastoris in order to obtain high - yield phytase - producing strain and to optimize the engineered yeast media recipes for the scale - up production of phytase . main results of this research are as follow : 1 , xba i - linized recombinant plasmid ppic9k - phya was transformed into pichia pastoris by gene pulser . 98 positive transformants showing measurable phytase activities were screened on md plates and ypd plates containing g418 . they all grew quickly on both md plates and mm plates , which proved their phenotypes of methanol utilization were mut + 主要实验结查如下：西南农业大学硕士学位论文1 、用乃ai酶切携带植酸酶基因表达片段的重组质粒ppicgk夕句) a ，回收dna ，用genepulser电击转化毕赤酵母，涂布md平板，又用含不同浓度g418的ypd平板进行抗性筛选，得到98个可检测到植酸酶活力的阳性转化子，它们在md 、 mm平板上均表现快速生长，说明其甲醇利用表型是mut干。

Similar Words：

"ypane mi river" English translation, "ypane river" English translation, "ypattern communication networkk" English translation, "ypbpr" English translation, "ypc" English translation, "ypd pile driver" English translation, "ypdi" English translation, "ype" English translation, "ype jhu" English translation, "ype-jhu" English translation